| 猪流感病毒多表位融合蛋白对小鼠的免疫原性分析 |
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| 引用本文： | 师小潇,哈卓,丁长根,潘洁,饶柏忠,刘惠莉.猪流感病毒多表位融合蛋白对小鼠的免疫原性分析[J].畜牧兽医学报,2011,42(9). |
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| 作者姓名： | 师小潇 哈卓 丁长根 潘洁 饶柏忠 刘惠莉 |
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| 作者单位： | 上海农业科学院畜牧兽医研究所/上海市农业遗传育种重点实验室,上海,201106 |
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| 基金项目： | 上海市科技委员会重点科技攻关项目(053919116) |
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| 摘 要： | 作者拟利用表位多肽的抗原性及黏膜佐剂免疫增强作用,设计可通过黏膜途径免疫接种的猪流感病毒通用型疫苗.体外合成H1N1、H3N2亚型猪流感病毒表位抗原基因,C末端串联大肠杆菌热敏性肠毒素LTb基因,构建pET30(a)-ep-LTb表达载体,利用SDS-PAGE、Western blot分析重组融合蛋白表达及生物学特性,小鼠免疫试验分析融合蛋白免疫原性.SDS-PAGE检测表达蛋白相对分子质量约38 ku,主要以包涵体形式存在.重组蛋白可与抗His-tag抗体和CTB抗体发生特异性反应.ELISA及HI试验检测,经黏膜途径免疫的小鼠产生了针对重组表位模拟抗原蛋白及H1N1、H3N2亚型猪流感病毒的血清抗体及局部黏膜分泌型IgA抗体.利用猪流感病毒表位抗原与LTb基因串连获得的融合蛋白具有良好的免疫原性和反应原性,且在黏膜接种局部产生理想的分泌型IgA抗体,能有效阻断病原由黏膜局部感染,为研制猪流感病毒通用型疫苗奠定了基础.
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| 关 键 词： | 猪流感病毒表位抗原 大肠杆菌热敏性肠毒素B亚基(LTb) 免疫原性 通用疫苗 |
Antigenicity of Fusion Protein Containing Swine Influenza Virus Multiple Epitopes in Mice |
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| SHI Xiao-xiao,HA Zhuo,DING Chang-gen,PAN Jie,RAOBai-zhong,LIU Hui-li.Antigenicity of Fusion Protein Containing Swine Influenza Virus Multiple Epitopes in Mice[J].Acta Veterinaria et Zootechnica Sinica,2011,42(9). |
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| Authors: | SHI Xiao-xiao HA Zhuo DING Chang-gen PAN Jie RAOBai-zhong LIU Hui-li |
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| Institution: | SHI Xiao-xiao,HA Zhuo,DING Chang-gen,PAN Jie,RAO Bai-zhong,LIU Hui-li (Shanghai Municipal Key Laboratory of Agricultural Genetics and Breeding,Institute of Animal Sciences and Veterinary Medicine,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China) |
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| Abstract: | Universal vaccine via intranasal immunization was designed based on antigenicity of the antigen epitope and the mucosal adjuvant of E.coli heat-labile toxin B subunit(LTb).Several epitopes of swine influenza virus(SIV) were designed and linked with LTb gene in C terminal.The recombinant pET30(a)-ep-LTb was constructed and the fusion protein was expressed in E.coli BL21(DE3).SDS-PAGE electrophoresis and Western blot were used for recombinant protein expression and bioactirity analysis.Humoral and mucosal ant... |
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| Keywords: | epitope of swine influenza virus heat-labile toxin B subunit(LTb) antigenicity universal vaccine |
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