| 蒙古绵羊Ghrelin基因的克隆和原核表达 |
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| 引用本文： | 梁建荣,曹贵方,赵鹏伟,温世勇,程兰玲,凃勇.蒙古绵羊Ghrelin基因的克隆和原核表达[J].畜牧兽医学报,2011,42(12):1782-1786. |
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| 作者姓名： | 梁建荣 曹贵方 赵鹏伟 温世勇 程兰玲 凃勇 |
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| 作者单位： | 内蒙古农业大学动物胚胎与发育生物学研究室,呼和浩特,010018 |
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| 基金项目： | 内蒙古自然科学基金项目(200711020506); 国家自然科学基金(30860201) |
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| 摘 要： | 为了克隆蒙古绵羊Ghrelin基因,构建其原核表达载体,并检测其在大肠杆菌中的表达情况.本研究用RT-PCR方法从蒙古绵羊胃组织mRNA扩增获得Ghrelin基因的cDNA序列,克隆到pMD-19T载体后进行序列分析.将测序正确的cDNA序列与原核表达载体pET-32a(+)连接,并转化BL21( DE3)大肠杆菌.经...
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| 关 键 词： | Ghrelin 基因克隆 原核表达 蒙古绵羊 |
The cDNA Cloning and Prokaryotic Expression of Mongolia Sheep Ghrelin Gene |
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| LIANG Jian-rong
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CAO Gui-fang
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ZHAO Peng-wei
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WEN Shi-yong
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CHENG Lan-ling
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TU Yong.The cDNA Cloning and Prokaryotic Expression of Mongolia Sheep Ghrelin Gene[J].Acta Veterinaria et Zootechnica Sinica,2011,42(12):1782-1786. |
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| Authors: | LIANG Jian-rong CAO Gui-fang ZHAO Peng-wei WEN Shi-yong CHENG Lan-ling TU Yong |
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| Institution: | LIANG Jian-rong,CAO Gui-fang,ZHAO Peng-wei,WEN Shi-yong,CHENG Lan-ling,TU Yong (Laboratory of Animal Embryo and Developmental Biology,Inner Mongolia Agricultural University,Huhhot 010018,China) |
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| Abstract: | To construct Mongolia sheep Ghrelin prokaryotic expression vector,and observe its expression in host Escherichia coli strain BL2l(DE3),the cDNA of Ghrelin gene was amplified from abomasum fundic gland mRNA of Mongolia sheep by RT-PCR.PCR product was cloned into the T vector pMD-19T to construct pMD-19T-Ghrelin for sequencing.Then the cDNA was subcloned into the prokaryotic expressing plasmid vector pET-32a(+) and transformed into host Escherichia coli strain BL2l(DE3)for expression.The clone was induced by ... |
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| Keywords: | Ghrelin gene cloning prokaryotic expression Mongolia sheep |
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