| β-catenin在不同毛色羊驼皮肤中的表达和定位 |
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| 引用本文： | 于秀菊,董常生,范阔海,贺俊平,姜俊兵,朱芷葳,董彦君.β-catenin在不同毛色羊驼皮肤中的表达和定位[J].畜牧兽医学报,2010,41(3). |
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| 作者姓名： | 于秀菊 董常生 范阔海 贺俊平 姜俊兵 朱芷葳 董彦君 |
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| 作者单位： | 山西农业大学动物科技学院,太谷,030801 |
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| 摘 要： | 旨在研究β-catenin在不同毛色羊驼皮肤中的表达和定位,探索其与毛色的关系。以成年白色和棕色羊驼为研究对象,采用实时荧光定量PCR技术、Western blot和免疫组织化学技术,对β-catenin在白色和棕色羊驼皮肤中mRNA、蛋白表达水平和定位进行研究。实时荧光定量PCR结果显示,β-catenin在棕色羊驼皮肤组织中的相对基因表达量是白色羊驼皮肤组织的1.662倍;Western blot结果显示,羊驼皮肤组织粗蛋白提取物中存在分子量约85 ku与兔抗β-catenin多克隆抗体发生免疫阳性反应的蛋白条带,棕色羊驼平均蛋白表达量显著高于白色羊驼;免疫组织化学结果显示,β-catenin在羊驼皮肤的表皮、毛乳头、毛根鞘和皮脂腺中表达,根据光密度值得出,除皮脂腺之外,在表皮、毛乳头和毛根鞘的表达差异极显著(P0.01)。结果提示β-catenin在白色和棕色羊驼皮肤组织中定位以及表达的差异,表明β-catenin参与毛色形成。
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| 关 键 词： | β-catenin 羊驼 实时荧光定量PCR 免疫组织化学 β-catenin |
Study on the Expression and Localization of β-catenin in Alpaca Skin of Different Colors |
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| YU Xiu-ju,DONG Chang-sheng,FAN Kuo-hai,HE Jun-ping,JIANG Jun-bing,ZHU Zhi-wei,DONG Yan-jun.Study on the Expression and Localization of β-catenin in Alpaca Skin of Different Colors[J].Acta Veterinaria et Zootechnica Sinica,2010,41(3). |
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| Authors: | YU Xiu-ju DONG Chang-sheng FAN Kuo-hai HE Jun-ping JIANG Jun-bing ZHU Zhi-wei DONG Yan-jun |
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| Institution: | YU Xiu-ju,DONG Chang-sheng,FAN Kuo-hai,HE Jun-ping,JIANG Jun-bing,ZHU Zhi-wei,DONG Yan-jun(College of Animal Science , Veterinary Medicine,Shanxi AgriculturalUniversity,Taigu 030801,China) |
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| Abstract: | The objective of this study was to study the expression and localization of β-catenin in alpaca skin of different colors,and to explore the function of β-catenin in the process of coat color formation.The mRNA and protein expression level of β-catenin in alpaca skin of different colors were examined by Real-time quantitative PCR,Western blot and immunohistochemistry.The gene expressive quantity of β-catenin in the brown alpaca's skin tissue was 1.662 times than ones of white alpaca.The distribution of β-catenin in alpaca skin were demonstrated,the expression was notable difference between the white and brown alpaca's based on the average optical density.Our findings showed that β-catenin was involved in the process of coat color formation. |
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| Keywords: | Western blot alpaca QRT-PCR Western blot immunohistochemistry |
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