| 猪源万古霉素耐药肠球菌分离及表型和基因型检测 |
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| 引用本文： | 俞道进,尹兵,易秀丽,马玉芳,李健,黄一帆.猪源万古霉素耐药肠球菌分离及表型和基因型检测[J].畜牧兽医学报,2011,42(2). |
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| 作者姓名： | 俞道进 尹兵 易秀丽 马玉芳 李健 黄一帆 |
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| 作者单位： | 福建农林大学动物科学学院,福州,350002 |
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| 基金项目： | 福建省自然科学基金项目(2009J0101); 福建省教育厅项目(2006F5003) |
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| 摘 要： | 本研究旨在调查3个猪场不同猪群粪便中万古霉素耐药肠球菌(VRE)的分离率并进行耐药表型、基因型的测定,为万古霉素耐药肠球菌的流行病学提供基础数据。根据美国临床和实验室标准协会(CLSI)推荐的方法进行耐药表型的测定,采用PCR法鉴定肠球菌并进行耐药基因的扩增,比较表型与基因型的一致性。结果从3个猪场250个粪便样本共分离出30株VRE。不同猪场不同猪群的分离率以哺乳仔猪最高,其次为保育仔猪、怀孕母猪和哺乳母猪,以大猪和公猪的分离率最低。耐万古霉素肠球菌均以土霉素的耐药率最高,为93.3%;以氨苄西林的敏感率最高,为80%。耐药基因检测2株为VanA阳性,12株为VanB阳性,7株为VanC1阳性,5株为VanC2/3阳性,4株未扩增出Van基因。本研究显示3个猪场均能分离到耐万古霉素肠球菌,各猪群耐药菌分离率与猪群接触抗菌药物的程度有一定的相关性,分离的VRE对6种抗生素具有不同程度的耐药,耐药表型与基因型基本一致。
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| 关 键 词： | 肠球菌 耐药 万古霉素 猪场 |
Isolation of Vancomycin Resistant Enterococci and Detection of Its Phenotype,Genotype from Pig Farms |
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| YU Dao-jin,YIN Bing,YI Xiu-li,MA Yu-fang,LI Jian,HUANG Yi-fan.Isolation of Vancomycin Resistant Enterococci and Detection of Its Phenotype,Genotype from Pig Farms[J].Acta Veterinaria et Zootechnica Sinica,2011,42(2). |
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| Authors: | YU Dao-jin YIN Bing YI Xiu-li MA Yu-fang LI Jian HUANG Yi-fan |
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| Institution: | YU Dao-jin,YIN Bing,YI Xiu-li,MA Yu-fang,LI Jian,HUANG Yi-fan(College of Animal Science of Fujian Agriculture and Forestry University,Fuzhou350002,China) |
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| Abstract: | This experiment was conducted to investigate the isolation ratio,the phenotype and the genotype of Vancomycin-resistant Enterococci(VRE) from 3 different pig farms and provide basic data for the epidemiology of VRE.A broth dilution method recommended by American Society of Clinical and Laboratory Standards Institute(CLSI) was used to determinate the resistant phenotypic,a PCR method was used to identify Enterococci and detect Vancomycin-resistant gene at the same time.The results were as follows: 30 strains... |
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| Keywords: | Enterococci resistance Vancomycin pig farm |
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