| 披针叶黄华内生真菌的分离与鉴定 |
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| 引用本文： | 赵文星,吴可欣,郭梓豫,张云昊,唐丽辉,刘奕伶,莫重辉,赵宝玉,路浩.披针叶黄华内生真菌的分离与鉴定[J].畜牧兽医学报,2021,52(10):2986-2994. |
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| 作者姓名： | 赵文星 吴可欣 郭梓豫 张云昊 唐丽辉 刘奕伶 莫重辉 赵宝玉 路浩 |
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| 作者单位： | 1. 西北农林科技大学动物医学院, 杨凌 712100;2. 青海大学农牧学院, 西宁 810016 |
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| 基金项目： | 国家自然科学基金（32072929）；青海省科技援青专项（2020-QY-210） |
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| 摘 要： | 旨在探讨有毒植物披针叶黄华内生真菌种类与种群分布情况，本试验以采自青海的披针叶黄华为试验材料，用表面消毒法对样品消毒后进行内生真菌分离，结合形态学鉴定和5.8S rDNA-ITS序列分析对分离菌株进行种属鉴定，并应用MEGA7.0软件进行系统发育分析。结果显示，从披针叶黄华中共分离获得29种内生真菌，分属于7纲、9目、11科、12属。披针叶黄华内生真菌总相对分离频率为79.31%，其中，叶的内生真菌种类最多（41.38%），茎次之（37.93%），种子（13.79%）和根（6.90%）相对较少；根据系统发育分析，所有内生真菌分属于两大种群，种群间亲缘关系较近。综上表明，披针叶黄华内生真菌种类丰富，青霉属是优势菌属。
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| 关 键 词： | 披针叶黄华 内生真菌 分离鉴定 系统发育分析 青霉属 |
| 收稿时间： | 2021-01-25 |
Isolation and Identification of Endophytic Fungi in Thermopsis lanceolata |
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| ZHAO Wenxing,WU Kexin,GUO Ziyu,ZHANG Yunhao,TANG Lihui,LIU Yiling,MO Chonghui,ZHAO Baoyu,LU Hao.Isolation and Identification of Endophytic Fungi in Thermopsis lanceolata[J].Acta Veterinaria et Zootechnica Sinica,2021,52(10):2986-2994. |
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| Authors: | ZHAO Wenxing WU Kexin GUO Ziyu ZHANG Yunhao TANG Lihui LIU Yiling MO Chonghui ZHAO Baoyu LU Hao |
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| Institution: | 1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China;2. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China |
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| Abstract: | To investigate the endophytic fungi species and population distribution of the poisonous plant, Thermopsis lanceolata, the experimental materials collected from Qinghai Province were disinfected with surface disinfection method and then endophytic fungi were separated. The morphological and 5.8S rDNA-ITS sequence analysis were used to identify these strains, and the MEGA7.0 software was used for phylogenetic analysis. The results showed that twenty-nine endophytic fungi were isolated from the Thermopsis lanceolata, which belong to 7 classes, 9 orders, 11 families and 12 genera. The total relative separation frequency was 85.42%. Endophytic fungi are most commonly isolated from leaves (41.38%), followed by stems (37.93%), seed (13.79%) and roots (6.90%). According to phylogenetic analysis, all endophytic fungi belong to two populations with close genetic relationship. The results indicated that Thermopsis lanceolata had abundant endophytic fungi species and Penicillium sp. was the dominant genus. |
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| Keywords: | Thermopsis lanceolata endophytic fungi isolation and identification phylogenetic analysis Penicillium sp |
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