| 新疆地区腹泻仔猪源大肠杆菌的分群、血清型鉴定及耐药性分析 |
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| 引用本文： | 佟盼盼,黄顺敏,王芋丹,施旭辉,陈文霞,宋鑫龙,张毅,苏战强,谢金鑫.新疆地区腹泻仔猪源大肠杆菌的分群、血清型鉴定及耐药性分析[J].畜牧兽医学报,2023,54(1):414-420. |
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| 作者姓名： | 佟盼盼 黄顺敏 王芋丹 施旭辉 陈文霞 宋鑫龙 张毅 苏战强 谢金鑫 |
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| 作者单位： | 新疆农业大学动物医学学院, 乌鲁木齐 830052 |
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| 基金项目： | 国家自然科学基金(31960695)；自治区高层次人才引进工程项目(XJGCC2018080)；新疆农业大学博士后科研流动站资助(214045)；2022年自治区研究生科研创新项目；新疆农业大学校级大学生创新创业训练计划项目(dxscx2022181) |
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| 摘 要： | 旨在了解新疆地区腹泻仔猪源大肠杆菌的系统进化分群、血清型及耐药性。本研究对154份腹泻仔猪粪便样品进行大肠杆菌的分离鉴定，采用多重PCR方法对分离株进行系统进化分群和O血清型鉴定，通过K-B纸片法对其进行药物敏感性检测并通过PCR方法进行耐药基因检测。结果显示：共分离到154株大肠杆菌，包括ETEC(n=24)、STEC(n=21)、EPEC(n=1)、EPEC/STEC(n=2)、ETEC/STEC(n=1)和ETEC/EPEC(n=1),其他104株。系统进化分群显示，多数菌株属于B1(37%)和A群(31%)。定型菌株44株，分别属于10种血清型，以O154、O12、O8、O141和O175为主要流行血清型。151株(98%)为多重耐药菌，对复方新诺明、四环素、氨苄西林、链霉素和氯霉素的耐药率为81%～100%,对阿莫西林/克拉维酸、头孢噻肟、庆大霉素、头孢曲松、环丙沙星和阿米卡星的耐药率为31%～66%,对左氧氟沙星、多黏菌素B、头孢他啶、头孢吡肟、氨苄西林-舒巴坦、哌拉西林-他唑巴坦和亚胺培南的耐药率为1%～19%。耐药基因tetA(88%)、tetG(60%)和cmlA(4...
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| 关 键 词： | 腹泻仔猪 大肠杆菌 系统进化分群 血清型 耐药性 |
| 收稿时间： | 2022-04-19 |
Phylogenetic Clustering,Serotype and Drug Resistance Analysis of Escherichia coli from Diarrhea with Piglets in Xinjiang |
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| TONG Panpan,HUANG Shunmin,WANG Yudan,SHI Xuhui,CHEN Wenxia,SONG Xinlong,ZHANG Yi,SU Zhanqiang,XIE Jinxin.Phylogenetic Clustering,Serotype and Drug Resistance Analysis of Escherichia coli from Diarrhea with Piglets in Xinjiang[J].Acta Veterinaria et Zootechnica Sinica,2023,54(1):414-420. |
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| Authors: | TONG Panpan HUANG Shunmin WANG Yudan SHI Xuhui CHEN Wenxia SONG Xinlong ZHANG Yi SU Zhanqiang XIE Jinxin |
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| Institution: | College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | This study was conducted to examine the distribution of phylogenetic clustering, virulence genes, serotypes and drug resistance of Escherichia coli (E. coli) from diarrhea with piglets in Xinjiang. In this study, 154 fecal samples were collected from diarrhea with piglets, and isolation, identification of E. coli was conducted. Phylogenetic clustering, virulence genes, serotype were tested by multiple PCR method. Drug sensitivity of isolates was determined by using a Kirby-Bauer disk diffusion method and drug resistance gene was detected by PCR. The results showed that 154 E. coli were isolated, including ETEC (n=24), STEC (n=21), EPEC (n=1), EPEC/STEC (n=2), ETEC/STEC (n=1), ETEC/EPEC (n=1), and other 104 strains. Phylotyping assays showed that most strains largely belong to group A (37%) and B1 (31%). O serogroups were identified for 44 E. coli isolates, of which O154, O12, O8, O141 and O175 were dominant serogroups. One hundred and fifty-one strains were multiple drug resistance (MDR), and the drug resistance rates of 154 E. coli isolates to cotrimoxazole, tetracycline, ampicillin, streptomycin and chloramphenicol ranged from 81% to 100%, that to amoxicillin/clavulanate, cefotaxime, gentamicin, ceftriaxone, ciprofloxacin and amikacin ranged from 31% to 66%, that to levofloxacin, polymyxins B, ceftazidime, cefepime, ampicillin -sulbactam, piperacillin -tazobactam and imipenem ranged from 1% to 19%. The prevalence of drug resistance genes tetA (88%), tetG (60%) and cmlA (45%) was higher, while blaCTX-M-2G, blaTEM, blaSHVand tetE were all lower than 30%. blaCTX-M-2G, blaTEM, blaSHV and tetE were not detected. The results indicated that the types of E. coli from diarrhea with piglets in Xinjiang were complex and the situation of MDR was severe, the prevalence of drug resistance genes was diversified, and important antibiotic resistance phenotypes were detected in human and clinical, so the monitoring of drug resistance of E. coli in pig farms should be strengthened. |
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| Keywords: | diarrhea with piglets Escherichia coli phylogenetic clustering serum group drug resistance |
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