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| 双黄连注射液对中性粒细胞磷酸二酯酶4B基因表达的影响 | |
| 引用本文： | 姜代勋,陈武,谷金妮,于同泉,路苹,穆祥.双黄连注射液对中性粒细胞磷酸二酯酶4B基因表达的影响[J].畜牧兽医学报,2007,38(11):1262-1266. |
| 作者姓名： | 姜代勋 陈武 谷金妮 于同泉 路苹 穆祥 |
| 作者单位： | 1. 北京农学院兽医学(中医药)北京市重点实验室,北京,102206 2. 北京农学院动物科学技术系,北京,102206 3. 北京农学院农业应用新技术北京市重点实验室,北京,102206 |
| 基金项目： | 国家自然科学基金项目(30671542)，北京市科技新星项目(9558100500)，北京市属市管高校人才强教计划资助项目 |
| 摘 要： | 本试验以主要在中性粒细胞中表达的磷酸二酯酶4B基因为靶基因,研究双黄连注射液的抗炎机制.采用半定量RT-PCR法检测双黄连注射液在不同时间对磷酸二酯酶4B基因表达的影响.结果发现双黄连注射液与中性粒细胞共孵育1 h时对磷酸二酯酶4B基因表达无明显的影响,当孵育到3 h时即可极显著抑制磷酸二酯酶4B基因的表达(P＜0.01).本试验结果表明,双黄连注射液能在基因转录水平上抑制中性粒细胞内磷酸二酯酶4B基因的表达,提示其能使cAMP含量升高而抑制中性粒细胞等炎症细胞的活化,从而发挥抗炎作用. |
| 关 键 词： | 磷酸二酯酶 中性粒细胞 双黄连注射液 |
| 文章编号： | 0366-6964（2007）11-1262-05 |
| 修稿时间： | 2006-10-18 |
Influence of Shuanghuanglian Injection on Phosphodiesterase 4B Gene Expression in Neutrophils |
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| JIANG Dai-xun,CHEN Wu,GU Jin-ni,YU Tong-quan,LU Ping,MU Xiang.Influence of Shuanghuanglian Injection on Phosphodiesterase 4B Gene Expression in Neutrophils[J].Acta Veterinaria et Zootechnica Sinica,2007,38(11):1262-1266. | |
| Authors: | JIANG Dai-xun CHEN Wu GU Jin-ni YU Tong-quan LU Ping MU Xiang |
| Institution: | 1. Beijing Key Laboratory of Veterinary Medicine (Traditional Chinese Medicine and Pharmacology |
| Abstract: | The influence of Shuanghuanglian Injection on phosphodiesterase 4B gene expression in neutrophils and it's anti-inflammatory mechanism in neutrophils were studied.The quantity of phosphodiesterase 4B gene expression was detected by semi-quantitative RT-PCR in different time to evaluated the influence of Shuanghuanglian Injection.Expression of phosphodiesterase 4B gene in neatrophils had no notable change after incubating with Shuanghuanglian Injection for 1 h,but significantly reduced(P<0.01) after incubating with Shuanghuanglian Injection for 3 h.The results suggested that Shuanghuanglian Injection can inhibit phosphodiesterase 4B gene expression in neutrophils in certain time,making cAMP content rise and inhibiting activation of inflammatory cells such as neutrophils,consequently,to exert the anti-inflammation. |
| Keywords: | phosphodiesterase neutrophils Shuanghuanglian Injection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |