| 盐旱胁迫对花花柴种子萌发与幼苗生理生化特性的影响 |
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| 引用本文： | 石新建,王彦芹,李志军.盐旱胁迫对花花柴种子萌发与幼苗生理生化特性的影响[J].草业科学,2017,34(9). |
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| 作者姓名： | 石新建 王彦芹 李志军 |
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| 作者单位： | 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,薪疆石河子832000;新疆农垦科学院,新疆石河子832000;新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,薪疆石河子832000;塔里木大学生命科学学院,新疆石河子832000 |
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| 基金项目： | 新疆生产建设兵团科技计划，国家自然科学基金 |
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| 摘 要： | 为探明花花柴(Karelinia caspica)种子萌发和幼苗对盐旱胁迫的耐受性,采用不同浓度的PEG-6000溶液和NaCl溶液模拟盐旱胁迫,研究盐旱胁迫对花花柴种子萌发及幼苗保护酶活性、相对电导率和丙二醛(MDA)含量的影响。结果表明,1)花花柴种子最终萌发率随着NaCl和PEG胁迫程度的升高而降低,低浓度胁迫下的种子最终萌发率与对照无显著差异(P0.05);当PEG质量分数大于20%、NaCl溶液浓度大于100mmol·L-1时,各处理下的种子最终萌发率差异显著(P0.05)。2)NaCl浓度为200~300mmol·L-1和PEG质量分数为5%~15%胁迫下的花花柴幼苗叶片丙二醛(MDA)含量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性随着胁迫时间延长逐渐升高;当幼苗在400~500mmol·L-1 NaCl和25%的PEG胁迫下时,叶片MDA含量随着胁迫时间延长逐渐升高;POD、SOD和CAT活性随着胁迫时间延长先升高后降低。可见,花花柴对逆境环境具有较强的适应能力;25%的PEG为其耐旱临界值,300mol·L-1 NaCl为其耐盐临界值。
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| 关 键 词： | 花花柴 种子萌发 幼苗 保护酶活性 丙二醛(MDA) NaCl胁迫 PEG胁迫 |
Physiological changes during seed germination and seedling development in Karelinia caspia Less.under drought and salinity stress |
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| Shi Xin-jian,Wang Yan-qin,Li Zhi-jun.Physiological changes during seed germination and seedling development in Karelinia caspia Less.under drought and salinity stress[J].Pratacultural Science,2017,34(9). |
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| Authors: | Shi Xin-jian Wang Yan-qin Li Zhi-jun |
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| Abstract: | In order to explore the physiological changes in Karelinia caspia during seed germination and seedling development under drought and salt stress,we used different concentrations of PEG-6000 or NaCl to simulate drought and salt stress,respectively.Germination rate,protective enzyme activity,relative conductivity and the Malondialdehyde (MDA)content during seed germination and in the seedling of K.caspia under stress (drought and salinity) were determined.The results show that:1) With increasing amounts of PEG and NaCl,a gradual decrease in germination was observed,while there were no significant differences between the seeds treated with 15％ PEG and 50 mmol · L-1 NaCl when compared to control.However,when PEG was above 20 ％,or when NaCl concentration was increased above 100 mmol · L-1,we observed significant differences between treated samples and control.2) In the range of 5％～20％ PEG or 200～400 mmol · L-1 NaCl,with a concentration gradient and increasing stress duration,a corresponding increase in MDA content,and elevated enzymatic activities for POD,SOD and CAT were observed.When plants were treated with 25％ PEG and 500 mmol · L-1 NaCl,the relative conductivity and MDA content increased significantly with prolonged stress periods.Enzyme activities for POD,SOD and CAT also increased initially with prolonged stress before decreasing to normal levels.These preliminary results reveal that K.caspia Less has huge potential to adapt to adverse environmental conditions.Also,our study revealed that the drought threshold was equivalent to the PEG 25 ％,while the salinity threshold was the equivalent of 300 mmol · L-1 NaCl. |
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| Keywords: | Karelinia caspia Less seed germination seedling protective enzyme activity mmalondialdehyde (MDA) NaCl stress PEG stress |
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