| 生驼乳中金黄色葡萄球菌的分离鉴定与耐药性分析 |
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| 引用本文： | 欧秀玲,蔡扩军,徐敏.生驼乳中金黄色葡萄球菌的分离鉴定与耐药性分析[J].中国乳业,2022,0(8):29-33. |
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| 作者姓名： | 欧秀玲 蔡扩军 徐敏 |
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| 作者单位： | 1 新疆华博检测技术有限公司,新疆乌鲁木齐 830000;2 新疆农业大学动物医学学院,新疆乌鲁木齐 830052;3 乌鲁木齐市动物疾病控制与诊断中心,新疆乌鲁木齐 830063;4 乌鲁木齐市奶业协会,新疆乌鲁木齐 830063 |
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| 基金项目： | 2022年新疆维吾尔自治区科技成果转化示范专项―科技特派员农村科技创业行动项目（2022KF138）; 新疆维吾尔自治区天山英才计划第三期; 新疆维吾尔自治区科技厅自然科学基金面上项目（2021D01A40） |
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| 摘 要： | 为了解生驼乳中金黄色葡萄球菌的污染状况,本文从骆驼养殖户中采集了90 份生驼乳,对其进行金黄色葡萄球菌的增菌、培养和分离,并利用全自动微生物生化鉴定仪进行鉴定,同时对分离出的阳性菌株采用纸片扩散法进行耐药性分析。生化鉴定结果表明,90 份生驼乳样本中分离出8 株金黄色葡萄球菌,分离率8.9%。耐药性分析表明,8 株阳性菌株对青霉素耐药率达75%;对氧氟沙星敏感率最高,可达100%;对头孢哌酮钠、环丙沙星敏感性较高,敏感率可达87.5%;对链霉素、卡那霉素、阿莫西林、头孢唑啉和头孢西丁,四环素、氯霉素、头孢噻肟、林可霉素、苯唑西林、庆大霉素较敏感,敏感率在37.5%~75.0%之间。本研究为对生驼乳中的金黄色葡萄球菌的污染防控提供了参考依据。
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| 关 键 词： | 生驼乳 金黄色葡萄球菌 分离鉴定 耐药分析 |
Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus from Raw Camel Milk |
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| OU Xiuling,CAI Kuojun,XU Min.Isolation,Identification and Drug Resistance Analysis of Staphylococcus aureus from Raw Camel Milk[J].China Dairy,2022,0(8):29-33. |
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| Authors: | OU Xiuling CAI Kuojun XU Min |
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| Institution: | 1Xinjiang Huabo Testing Technology Co.,Ltd.,Urumqi Xinjiang 830052;2Xinjiang Agriculture University,Urumqi Xinjiang 830052;3Urumqi Animal Disease Control and Diagnosis Center,Urumqi Xinjiang 830063;4Urumqi Dairy Association,Urumqi Xinjiang 830063 |
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| Abstract: | In order to understand the contamination status of Staphylococcus aureus in raw camel milk in the breeding process,this study collected 90 raw camel milk samples from camel farmers,and carried out the enrichment,culture and separation of Staphylococcus aureus,and the automatic microbial biochemical identification instrument was used for identification,and the drug resistance analysis of the isolated positive strains was carried out by the disc diffusion method. The results of biochemical identification showed that 8 strains of Staphylococcus aureus were isolated from 90 raw camel milk samples, with an isolation rate of 8.9%. The drug resistance analysis showed that the 8 positive strains were resistant to penicillin up to 75%,the sensitivity rate to ofloxacin was the highest,up to 100%,the sensitivity rate to cefoperazone sodium and ciprofloxacin was higher,and the sensitivity rate was up to 87.5%,the sensitivity rate to streptomycin,kanamycin, amoxicillin,cefazolin and cefoxitin,tetracycline,chloramphenicol, cefotaxime,lincomycin,benzacillin and gentamicin ranged from 37.5% to 75.0%. This study provides a reference for the prevention and control of Staphylococcus aureus in raw camel milk. |
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| Keywords: | raw camel milk Staphylococcus aureus isolation and identification drug resistance analysis |
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