| 新疆部分地区奶牛乳房炎大肠杆菌的分离鉴定及耐药性分析 |
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| 引用本文： | 贾立敏,蔡扩军.新疆部分地区奶牛乳房炎大肠杆菌的分离鉴定及耐药性分析[J].中国乳业,2022,0(12):58-62. |
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| 作者姓名： | 贾立敏 蔡扩军 |
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| 作者单位： | 1 乌鲁木齐市动物疾病控制与诊断中心,新疆乌鲁木齐 830063; 2 新疆农业大学动物医学学院,新疆乌鲁木齐 830052 |
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| 基金项目： | 新疆维吾尔自治区科技厅自然科学基金面上项目（2021D01A40）; 新疆维吾尔自治区天山英才计划第三期 |
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| 摘 要： | 目的]为了解本地区奶牛乳房炎大肠杆菌的流行及耐药情况。方法]对新疆部分地区奶牛乳房炎大肠杆菌进行分离培养、染色镜检、荧光PCR鉴定,并用纸片法对分离菌株开展药物敏感试验。结果]从200 份奶牛乳房炎样品中分离出63 株大肠杆菌,分离率为31.5%。分离菌株对青霉素耐药率最高,达到了93.65%,对美罗培南敏感率最高,达到了98.14%,对其他抗生素均有不同程度的耐药。结论]新疆部分地区奶牛乳房炎中大肠杆菌广泛流行,具有较高的耐药性。
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| 关 键 词： | 奶牛乳房炎 大肠杆菌 分离鉴定 荧光PCR 耐药性 |
Isolation,Identification and Drug Resistance Analysis of Escherichia coli from Dairy Cow Mastitis in Some Areas of Xinjiang |
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| JIA Limin,CAI Kuojun.Isolation,Identification and Drug Resistance Analysis of Escherichia coli from Dairy Cow Mastitis in Some Areas of Xinjiang[J].China Dairy,2022,0(12):58-62. |
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| Authors: | JIA Limin CAI Kuojun |
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| Institution: | 1Urumqi Animal Disease Control and Diagnosis Center,Urumqi Xinjiang 830063; 2Xinjiang Agriculture University,Urumqi Xinjiang 830052 |
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| Abstract: | Objective]In order to understand the prevalence and drug resistance of Escherichia coli in dairy cow mastitis in Xinjiang. Method]The Escherichia coli from dairy cow mastitis in some areas of Xinjiang were isolated,cultured,stained and identified by fluorescent PCR. The drug sensitivity test of the isolated strains was carried out by disk method. Result]63 strains of Escherichia coli were isolated from 200 dairy cow mastitis samples,and the isolation rate was 31.5%. The isolates were most resistant to penicillin （93.65%） and most sensitive to meropenem （98.14%）.The isolates were resistant to other antibiotics in different degrees. Conclusion]The results showed that Escherichia coli of dairy cow mastitis was widespread and had high drug resistance in some areas of Xinjiang. |
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| Keywords: | dairy cow mastitis Escherichia coli isolation and identification fluorescence PCR drug resistance |
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