Venezuelan equine encephalomyelitis virus: Concentration,partial purification,inactivation and immunogenicity |
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| Authors: | NM Foster TL Barber TE Walton |
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| Institution: | U.S. Department of Agriculture, Agricultural Research Service, Arthropodborne Animal Disease Research Laboratory, P.O. Box 25327, Denver Federal Center, Denver, CO 80225, U.S.A. |
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| Abstract: | Venezuelan equine encephalomyelitis (VEE) TC-84 vaccinal virus, from 10-1. quantities of infected duck embryo fibroblast cell culture fluids, was isolated by combined continuous-flow centrifugation with isopycnic banding in sucrose. Most of the recovered infectivity and hemagglutinating activity were in a single band at a buoyant density (?) of 1.2. About 90% of the total input protein (450–520 mg) was removed with the effluent, whereas most of the remaining 10% also banded at a ? of 1.2. Infectivity was inactivated with formalin at a final concentration of 0.05% at 37°C for 24 hr. Formalin-inactivated virus retained its immunogenicity and induced VEE virus-specific antibody in horses and guinea pigs. The horses and those guinea pigs that received equivalent doses of vaccine survived after a challenge of their immunity with virulent VEE virus. |
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| Keywords: | Venezuelan equine encephalomyelitis virus inactivated vaccine Virus de l'encéphalomyélite équine vénézuélienne vaccin inactivé |
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