Diversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes |
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| Institution: | 1. Pós-graduação em Microbiologia Agropecuária, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;2. Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;3. Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz/FioCruz, Rio de Janeiro, RJ, Brazil;4. Direcção de Ciências Animais, Maputo, Moçambique;1. Faculty of Medicine, Semmelweis University, Szentkirályi Street 46, 1088 Budapest, Hungary;2. 2nd Department of Internal Medicine, Semmelweis University, Szentkirályi Street 46, 1088 Budapest, Hungary;1. Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz (UESC), Campus Soane Nazaré de Andrade Rodovia, Ilhéus Itabuna, Km 16, Salobrinho, Ilhéus, Bahia 45662-000, Brazil;2. Departamento de Patologia Animal, Universidade Estadual Julio Mesquita Filho, UNESP/Jaboticabal, Rod. Paulo Castelanne s/n, Jaboticabal, São Paulo 14884-900, Brazil;3. Departamento de Zootecnia, Universidade Estadual Julio Mesquita Filho, UNESP/Jaboticabal, Rod. Paulo Castelanne s/n, Jaboticabal, São Paulo 14884-900, Brazil;1. Universidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabal, SP, Brazil;2. Johns Hopkins School of Medicine, Baltimore, Maryland, USA;3. Uniformed Services University, Bethesda, Maryland, USA;4. Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, Brazil;5. Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, Brazil;6. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Instituto Oswaldo Cruz/Fiocruz, Rio de Janeiro, Brazil;1. Departament of Preventive Veterinary Medicine, Universidade Estadual de Londrina, Londrina, PR, 86057-970, Brazil;2. Laboratory of Animal Health, Instituto Federal do Maranhão, São Luís, MA, 65095-460, Brazil;3. Departament of Veterinary Medicine, Universidade Federal do Paraná, Curitiba, PR, 80035-050, Brazil;1. Department of Veterinary Clinical Pathology, School of Veterinary Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;2. Department of Comparative Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN, USA;3. School of Agronomy and Veterinary Medicine, Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil;4. Department of Preventive Veterinary Medicine, School of Veterinary Medicine, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil;5. Department of Veterinary Clinical Pathology, Universidade Federal do Paraná, Curitiba, Paraná, Brazil |
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| Abstract: | Although hemoplasma infection in domestic animals has been well documented, little is known about the prevalence and genetic diversity of these bacteria in wild rodents. The present work aimed to investigate the occurrence of hemotrophic mycoplasmas in wild rodents from five Brazilian biomes, assessing the 16S rRNA phylogenetic position of hemoplasma species by molecular approach. Spleen tissues were obtained from 500 rodents, comprising 52 different rodent species trapped between 2000 and 2011. DNA samples were submitted to previously described PCR protocols for amplifying Mycoplasma spp. based on 16S rRNA, followed by sequencing and phylogenetic inferences. Among 457 rodent spleen samples showing absence of inhibitors, 100 (21.9%) were PCR positive to Mycoplasma spp. The occurrence of hemotropic mycoplasmas among all sampled rodents was demonstrated in all five biomes and ranged from 9.3% (7/75) to 26.2% (38/145). The Blastn analysis showed that amplified sequences had a percentage of identity ranging from 86 to 99% with other murine hemoplasmas. The ML phylogenetic analysis of 16S rRNA gene of 24 positive randomly selected samples showed the presence of ten distinct groups, all clustering within the Mycoplasma haemofelis. The phylogenetic assessment suggests the circulation of novel hemoplasma species in rodents from different biomes in Brazil. |
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| Keywords: | Hemotropic mycoplasmas Wild rodents Molecular characterization Brazil |
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