Identification of feline blood B and T lymphocytes by cell electrophoresis. |
| |
| Authors: | F Dumont E Reichart |
| |
| Institution: | 1. Unit of Experimental Cancerology, INSERM — U.95 Vandoeuvre-lès-Nancy, France;3. Unit of Respiratory Pathophysiology, INSERM — U.14 Vandoeuvre-lès-Nancy, France |
| |
| Abstract: | The electrokinetic properties of feline blood lymphocytes isolated by centrifugation over Ficoll-Isopaque gradient were investigated. A biphasic electrophoretic mobility (EPM) distribution was regularly observed with a low-mobility (LM) population (mean EPM: 0.82) accounting for 32% of blood lymphocytes and a high-mobility (HM) population (mean EPM: 1.09) representing 68% of blood lymphocytes. Following fractionation on nylon-wool columns, lymphocytes with B-cell properties (64% sIg+; 9% guinea pig erythrocytes (GPE)-rosette+, PHA and Con A unresponsive) were enriched in the adherent fraction and belonged mainly (78%) to the LM population. In contrast, lymphocytes with T properties (5% sIg+, 42% GPE-rosette+, PHA and Con A responsive) were recovered in the effluent fraction and comprised 84% of HM elements.Thus, in cat blood, LM lymphocytes are likely to represent in majority B cells and HM lymphocytes T cells. This indicates that cell electrophoresis provides an interesting mean for differentiating B and T cells in the cat. |
| |
| Keywords: | Cat blood lymphocytes (EPM) electrophoretic mobility Chat sang lymphocytes mobilité électrophorétique (MEP) |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
