| 酵母菌和乳酸菌发酵葡萄皮渣生物饲料的研究 |
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| 引用本文： | 翟羽佳,张惠玲,张丽芝.酵母菌和乳酸菌发酵葡萄皮渣生物饲料的研究[J].畜牧与饲料科学,2018,39(7):70-70. |
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| 作者姓名： | 翟羽佳 张惠玲 张丽芝 |
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| 作者单位： | [1]宁夏大学,宁夏银川750021;[2]银川能源学院,宁夏银川750105 |
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| 基金项目： | 宁夏高等学校科学技术研究项目(NGY2017249)。 |
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| 摘 要： | 以葡萄皮渣为主要原料,探讨以产朊假丝酵母菌和嗜酸乳杆菌发酵葡萄皮渣制备生物饲料的最佳方法。采用正交试验方法,以发酵产物的真蛋白含量为指标,筛选最佳发酵工艺条件。结果表明,确定的产朊假丝酵母菌与嗜酸乳杆菌最佳接种比例为1.5∶1.0,接种量为10.0%;固体培养基配方为:葡萄皮渣75%,辅料25%(玉米∶麸皮=1∶1),尿素1.5%,硫酸铵1.5%,硫酸镁0.4%,磷酸二氢钾1.5%;最佳发酵条件为:发酵料投放量100g/500mL,料水比1.0∶1.0,自然条件下pH值,32℃发酵72h。在该条件下,发酵终产物的真蛋白含量为14.45%(干基),比发酵前提高4.35%。研究结果为葡萄皮渣的饲料化利用以及新饲料资源的开发利用提供了新思路。
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Preparation of Biological Feed with Grape Pomace Fermented by Yeast and Lactobacillus |
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| Abstract: | The objective of this study was to investigate the optimum preparation method of biological feed with grape pomace fermented by co-culture of Candida utilis and Lactobacillus acidophilus.Using true protein content of the fermentation products as evaluation index,the optimal fermentation conditions were screened by orthogonal experimental design method.The results showed that the optimum inoculation ratio of Candida utilis and Lactobacillus acidophilus was 1.5∶1.0 and the optimum inoculation amount was 10.0%;the formula of the solid fermentation medium were as followed:grape pomace 75%,auxiliary material 25%(corn∶wheat bran=1∶1),urea 1.5%,ammonium sulfate 1.5%,magnesium sulfate 0.4%,and potassium dihydrogen phosphate 1.5%;the optimum fermentation conditions were as followed:the quality of fermentation material was 100 g/500 mL,material-water ratio was 1.0∶1.0,fermentation at 32℃for 72 h with nature pH value.Under this condition,the true protein content of final fermentation products was 14.45%(dry matter),which was 4.35%higher than the raw material.Our results provide new ideas for feeding utilization of grape pomace and development of application of novel feed resources. |
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