| 河南省鸡源大肠杆菌的分离鉴定与耐药性分析 |
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| 引用本文： | 艾伟昌,方忠意,李金磊,董鹏,狄元冉,高延玲,吴志明,王璐,刘振阳.河南省鸡源大肠杆菌的分离鉴定与耐药性分析[J].畜牧与饲料科学,2018,39(1):4-4. |
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| 作者姓名： | 艾伟昌 方忠意 李金磊 董鹏 狄元冉 高延玲 吴志明 王璐 刘振阳 |
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| 作者单位： | [1]河南省野生动物救护中心,河南郑州450044;[2]河南省兽药饲料监察所,河南郑州450008;[3]河南农业大学牧医工程学院,河南郑州450002 |
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| 摘 要： | 为了掌握河南省鸡源大肠杆菌的耐药性情况,从规模化鸡场抽取样品120批,通过细菌的分离、纯化,采用BD Phoenix^TM-100全自动微生物鉴定系统对分离的大肠杆菌进行鉴定,并通过微量肉汤稀释法对分离菌株进行药物敏感性分析。结果显示,共分离菌株100株,分离率为83.3%。分离菌株对四环素、氨苄西林、磺胺异噁唑、恩诺沙星、复方新诺明耐药严重,耐药率均在80%以上,表明河南省鸡源大肠杆菌耐药情况较为严重。
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Isolation,Identification and Antimicrobial Resistance of Escherichia coli from Chicken in Henan Province |
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| Abstract: | The aim of the present study was to characterize the antimicrobial resistance profile of Escherichia coli（E. coli）isolates from chicken in Henan province. A total of 120 samples were recovered from the large-scale chicken farm, and the E.coli stains were isolated and subsequently confirmed by a commercial automated microbiology identification system（BD Phoenix^TM-100）. The susceptibility of the E. coli isolates to the selected antimicrobial agents was determined by broth micro-dilution test. The results showed that a total of 100 E. coli isolates were obtained with the isolating rate of 83.3%; high resistance rate（above 80%） were observed to tetracycline, ampicillin, sulfafurazole, enrofloxacin, and compound sulfamethoxazole. Our investigation demonstrates the prevalence of antibiotic resistant E. coli isolates in chicken farms in Henan Province. |
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