| 人绒毛膜促性腺激素对母驴促排卵效果的研究 |
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| 引用本文： | 人绒毛膜促性腺激素对母驴促排卵效果的研究.人绒毛膜促性腺激素对母驴促排卵效果的研究[J].畜牧与饲料科学,2021,42(3):59-65. |
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| 作者姓名： | 人绒毛膜促性腺激素对母驴促排卵效果的研究 |
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| 作者单位： | 1.内蒙古农业大学,内蒙古 呼和浩特 010018; 2.内蒙古自治区农牧业科学院,内蒙古 呼和浩特 010031 |
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| 基金项目： | 内蒙古农牧业创新基金项目“人绒毛膜促性腺激素促进驴卵泡排卵的应用研究”（2019CXJJM03） |
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| 摘 要： | 目的]研究人绒毛膜促性腺激素（human chorionic gonadotropin,hCG）对母驴卵泡发育、排卵率、受胎率以及血清生殖激素水平的影响。方法]选择优势卵泡直径在30~35 mm以及大于35 mm的母驴各30头,不同优势卵泡直径的母驴群体分别设置1个500 IU/头hCG处理组（n=10）、1个1 000 IU/头hCG处理组（n=10）、1个不接受hCG处理的对照组（n=10）。采用肌肉注射方法对各组母驴进行hCG处理。每隔24 h进行1次B超检查,观察各组母驴卵泡发育情况,测量卵泡直径;记录各组发生排卵的母驴数量,计算排卵率。对各组母驴进行人工输精,输精后第18天进行孕检,记录各组受胎母驴头数,计算各组受胎率。于hCG处理后0、24、48、72 h分别测定各组母驴血清中雌二醇（estradiol,E2）和孕酮（progesterone,PROG）水平。结果]2个群体母驴的卵泡直径随hCG注射剂量的增加而增大;优势卵泡直径大于35 mm的母驴群体中,肌肉注射hCG的2个组在处理后24 h内均出现排卵,而对照组母驴没有排卵;优势卵泡直径不同的2个母驴群体,在hCG处理48 h后排卵母驴数和排卵率与对照组相比均有所提高,其中,hCG处理后72 h,优势卵泡直径大于35 mm的母驴群体中,1 000 IU/头 hCG处理组的排卵率达到100%。2个母驴群体中,接受hCG处理的母驴,受胎率均高于对照,并且随hCG剂量的增加,受胎率有所提高;优势卵泡直径大于35 mm的母驴群体中,1 000 IU/头 hCG处理组的受胎率达到50%。2个母驴群体中,1 000 IU/头处理组在hCG处理后24 h的血清E2浓度均较0 h时有较大幅度的提升,在0~72 h内血清PROG浓度的总体提升幅度较大。结论]hCG处理可提高母驴的排卵率、受胎率以及血清中E2和PROG水平,1 000 IU/头剂量的效果更好。
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| 关 键 词： | 母驴 人绒毛膜促性腺激素 优势卵泡 排卵 |
| 收稿时间： | 2019-12-12 |
Efficacy of Human Chorionic Gonadotropin for Inducing Ovulation in Jenny |
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| Tangsiga,HAN Wulantuya,Narenhua,MA Yue-jun,ZHAO Jun-li,WU Hai-qing.Efficacy of Human Chorionic Gonadotropin for Inducing Ovulation in Jenny[J].Animal Husbandry and Feed Science,2021,42(3):59-65. |
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| Authors: | Tangsiga HAN Wulantuya Narenhua MA Yue-jun ZHAO Jun-li WU Hai-qing |
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| Institution: | 1. Inner Mongolia Agricultural University,Hohhot 010018,China; 2. Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China |
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| Abstract: | Objective] To assess the effects of human chorionic gonadotropin （hCG） on follicular development, ovulation rate, pregnancy rate and serum reproductive hormone level in jenny.Method] A total of 60 jennies with dominant follicle diameters of 30-35 mm （n=30） and larger than 35 mm （n=30） were selected. For either of the two jenny herds with different dominant follicle diameters, a 500 IU/head hCG treatment group （n=10）, a 1 000 IU/head hCG treatment group （n=10）, and a control group （n=10） without hCG treatment, were set respectively. The experimental jennies received hCG treatment by intramuscular injection. B-ultrasound examination were carried out every 24 h to observe the follicular development status, and the follicle diameters were determined. The heads of ovulated jennies were recorded, and the ovulation rate was calculated. Artificial insemination was performed. Pregnant examination was carried out on 18th day after insemination. The heads of pregnant jennies were recorded, and the pregnancy rate was calculated. The serum levels of estradiol （E2） and progesterone （PROG） were measured at 0, 24, 48 and 72 h after hCG treatment. Result] In both of the jenny herds, the follicle diameters were increased with the elevation of hCG dosage; for the jennies with dominant follicle diameters larger than 35 mm, the two treatment groups ovulated within 24 h after receiving intramuscular injection of hCG, while no ovulation was observed in the control group; in both of the jenny herds, the ovulated heads and ovulation rate of the treatment groups were increased compared with their corresponding control groups at 48 h after hCG treatment; for the jennies with dominant follicle diameters larger than 35 mm, the ovulation rate reached up to 100% at 72 h after 1 000 IU/head hCG treatment; in both of the jenny herds, the conception rates of the hCG treated jennies were higher than those of the controls, and showed an increasing trend with the elevation of hCG dosage. For the jennies with dominant follicle diameters larger than 35 mm, the pregnancy rate reached up to 50% at 72 h after 1 000 IU/head hCG treatment. In both of the jenny herds, the serum E2 levels were largely increased at 24 h after 1 000 IU/head hCG treatment compared with that at 0 h, and the serum PROG levels also exhibited an overall increasement during 0 to 72 h. Conclusion] Intramuscular injection of hCG improves the ovulation rate, pregnancy rate and serum levels of E2 and PROG of jenny, and the dosage of 1 000 IU/head has better efficacy. |
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| Keywords: | jenny human chorionic gonadotropin dominant follicle ovulation |
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