| 2017年上海、河北地区奶牛乳房炎克雷伯菌分离鉴定及耐药性分析 |
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| 引用本文： | 年上海、河北地区奶牛乳房炎克雷伯菌分离鉴定及耐药性分析.2017年上海、河北地区奶牛乳房炎克雷伯菌分离鉴定及耐药性分析[J].畜牧与饲料科学,2019,40(1):108-112. |
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| 作者姓名： | 年上海、河北地区奶牛乳房炎克雷伯菌分离鉴定及耐药性分析 |
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| 作者单位： | [1]内蒙古农牧业科学院资源环境与检测技术研究所,内蒙古呼和浩特010031;[2]农业农村部农产品质量安全监督检验测试中心(呼和浩特),内蒙古呼和浩特010031;[3]宁城县农牧业局,内蒙古宁城024200 |
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| 基金项目： | 国家农产品质量安全风险评估重大专项“生鲜乳病原微生物摸底排查与关键控制点评估”(GJFP201700803)。 |
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| 摘 要： | 为了摸清上海和河北地区奶牛乳房炎的主要致病菌克雷伯菌的分离率及其耐药情况,对采集自上海和河北两地的乳房炎患病奶牛乳样进行克雷伯菌的分离培养和形态学、分子生物学鉴定,并选择临床常用的抗生素对分离得到的克雷伯菌进行药敏试验。经革兰染色、镜检以及分子生物学鉴定发现,采集自上海地区的100份乳房炎患病奶牛乳样中有18份乳样存在克雷伯菌,分离率为18%;采集自河北地区的100份乳房炎患病奶牛乳样中有14份乳样存在克雷伯菌,分离率为14%;且上海和河北地区2017年下半年乳房炎患病奶牛乳样中克雷伯菌分离率均高于上半年。药敏试验结果表明,该试验分离得到的克雷伯菌对所测试的抗生素存在不同程度的耐药性,其中,单一耐药菌株占21.88%,多重耐药菌株占53.13%,全部敏感的菌株占12.50%。由该试验结果可以得出,上海、河北地区奶牛发生的乳房炎是与克雷伯菌感染有关;且分离得到的克雷伯菌对临床常用的抗生素存在不同程度的耐药性。在动物生产和兽医临床上应及时监控克雷伯菌的流行趋势和耐药性变迁,并合理使用抗生素以减少耐药菌株的产生。
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| 关 键 词： | 乳房炎 克雷伯菌 分离率 耐药性 奶牛 |
| 收稿时间： | 2018-06-19 |
Isolation,Identification and Antimicrobial Resistance Profiles of Klebsiella Associated with Dairy Cow Mastitis in Shanghai and Hebei Regions in 2017 |
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| FENG Xiao-hui,DU Lin,WANG Li-fang,ZHANG San-fen,SONG Jie,HU Ze-guang.Isolation,Identification and Antimicrobial Resistance Profiles of Klebsiella Associated with Dairy Cow Mastitis in Shanghai and Hebei Regions in 2017[J].Animal Husbandry and Feed Science,2019,40(1):108-112. |
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| Authors: | FENG Xiao-hui DU Lin WANG Li-fang ZHANG San-fen SONG Jie HU Ze-guang |
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| Institution: | [1]Institute of Resources,Environment and Detection Technology,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;[2]The Supervision,Inspection and Testing Center for Agricultural Product Quality(Hohhot),Ministry of Agriculture and Rural Affairs,Hohhot 010031,China;[3]Agricultural and Animal Husbandry Bureau of Ningcheng County,Ningcheng 024200,China |
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| Abstract: | The objective of this study was to determine the prevalence of Klebsiella isolates associated with dairy cow mastitis from Shanghai and Hebei regions and to characterize the antimicrobial resistance profiles of the isolates.Milk samples were recovered from the dairy cows with mastitis in Shanghai and Hebei regions and were tested for the presence of Klebsiella strains by traditional isolation and selective cultivation methods.Subsequently,the isolates were phenotypically and molecularly identified.The antimicrobial resistance of the isolates against the commonly used antibiotics in veterinary clinical practice was characterized.Among the 100 milk samples collected from Shanghai region,18 of them were Klebsiella positive,with a separation rate of 18%.For the 100 milk samples recovered from Hebei region,there were 14 milk samples with the presence of Klebsiella strains,indicating an isolating rate of 14%.A higher separation rate of Klebsiella was observed in the latter half of 2017 for both Shanghai and Hebei regions compared to the first half.The Klebsiella isolates expressed resistance to the tested antibiotics in different degrees.The isolates resistant to single one and multiple antimicrobial agents accounted for 21.88% and 53.13% of all strains,respectively.Only 12.50% of the isolates were susceptible to all of the tested antibiotics.Our results demonstrated that the presence of Klebsiella was associated with the emergence of dairy cow mastitis in Shanghai and Hebei regions,and the prevalence of drug resistant isolates were observed.The surveillances for the epidemiological trends and patterns of antimicrobial resistance of Klebsiella in animal production and veterinary clinical practice are urgently needed.Furthermore,prudent used of antibiotics in veterinary medicine is also strongly recommended to restrain the emergence of drug resistant bacteria. |
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