| 16味蒙药对3种仇清型牛源致病性大肠杆菌体外抑菌及联合抑菌效果的研究 |
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| 引用本文： | 味蒙药对种仇清型牛源致病性大肠杆菌体外抑菌及联合抑菌效果的研究.16味蒙药对3种仇清型牛源致病性大肠杆菌体外抑菌及联合抑菌效果的研究[J].畜牧与饲料科学,2018,39(10):102-107. |
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| 作者姓名： | 味蒙药对种仇清型牛源致病性大肠杆菌体外抑菌及联合抑菌效果的研究 |
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| 作者单位： | [1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018;[2]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018 |
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| 基金项目： | 国家自然科学基金项目（31260590）;国家科技支撑项目（2015BAD29806）. |
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| 摘 要： | 为研究蒙药对3种血清型牛源致病性大肠杆菌（Escherichia coli，E. coli）的体外抑菌效果，采用牛津杯法测定16味蒙药对受试菌株的抑菌圈直径，利用微量稀释法结合平板法测定最小抑菌浓度（MIC）和最低杀菌浓度（MBC），采用棋盘法测定敏感蒙药的协同指数（FICI）。结果表明，乌里史古—达兰—哈力苏、协日—洪连、乌拉勒吉嘎纳对3种血清型大肠杆菌的抑菌圈直径为16.10-29.64 mm，MIC为7.81-31.25 mg/mL，MBC为15.63-125 mg/mL。联合抑菌试验结果表明，乌拉勒吉嘎纳与协日—洪连，舒布格日—希木勒德格与呼仍—图如对E. coli O26的FICI为0.50，乌拉勒吉嘎纳与乌里史古—达兰—哈力苏对E. coli O83的FICI为0.50。结果提示，乌里史古—达兰—哈力苏、协日—洪连、呼仍—图如、乌拉勒吉嘎纳、阿纳尔对3种血清型大肠杆菌的综合抑菌效果较好；舒布格日—希木勒德格与呼仍—图如在抑制E. coli O26时表现为协同作用，组方时可优先考虑；乌拉勒吉嘎纳与乌里史古—达兰—哈力苏在抑制E. coli O83时表现为协同作用，组方中可优先考虑。
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| 关 键 词： | 蒙药 致病性大肠杆菌 血清型 体外抑菌 MIC MBC FICI |
| 收稿时间： | 2018-08-22 |
Antibacterial Effects and Combined Bacteriostatic Effects of 16 Kinds of Mongolian Herb Medicines against Bovine Pathogenic Escherichia coli of 3 Serotypes in Vitro |
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| GAO Rui-juan,WANG Chun-jie,Aorigele,GAO Si-ming.Antibacterial Effects and Combined Bacteriostatic Effects of 16 Kinds of Mongolian Herb Medicines against Bovine Pathogenic Escherichia coli of 3 Serotypes in Vitro[J].Animal Husbandry and Feed Science,2018,39(10):102-107. |
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| Authors: | GAO Rui-juan WANG Chun-jie Aorigele GAO Si-ming |
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| Institution: | [1]College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;[2]College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China |
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| Abstract: | The purpose of this study was to evaluate the antibacterial effects of 16 Mongolian herb medicines on pathogenic Escherichia coli （E. coli） of 3 different serotypes in vitro. The inhibition diameters of the Mongolian herb medicines against the tested strains were determined by Oxford cup method; the minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） were determined using micro-dilution method in combination with agar plate method; the fractional inhibitory concentration indexes（FICI） of the screened Mongolian herb medicines with superior antibacterial activity were determined by chessboard method. The results showed that the inhibition diameters of Urishgu-Dalan-Harrisu, Syner-Honglian and Ullajigana against the E. coli of 3 different serotypes ranged from 16.10 mm to 29.64 mm,the corresponding MIC ranged from 7.81 mg/mL to 31.25 mg/mL,and the corresponding MBC ranged from 15.63 mg/mL to 125 mg/mL; the FICI of Ullajigana and Syner-Honglian as well as Schubugri-Himuledeg and Hur-Turu against E. coli O26 were both 0.50; the FICI of Ullajigana and Urishgu-Dalan-Harrisu against E. coli O83 was 0.50. The combined data suggested that Urishgu-Dalan-Harrisu, Syner-Honglian, Hur-Turu, Ullajigana and Anar had a better performance in comprehensive bacteriostasis effect against E. coli of 3 serotypes; Schubugri-Himuledeg and Hur-Turu exhibited a synergistic effect in inhibiting E. coli O26, which could be given priority in the formulation; Ullajigana and Urishgu-Dalan-Harrisu expressed a synergistic effect in inhibiting E. coli O83, which could be given priority in the formulation. |
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