| 布鲁菌疫苗株Rev.1全基因序列的测定与分析 |
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| 引用本文： | 于环宇,张志丹,刘建奇,赵丹彤,赵丽霞,刘国英,宋庆庆,王国华,潘相臣,刘冬冬,田普厚,石顺利,关平原.布鲁菌疫苗株Rev.1全基因序列的测定与分析[J].畜牧与饲料科学,2020,41(5):91-95. |
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| 作者姓名： | 于环宇 张志丹 刘建奇 赵丹彤 赵丽霞 刘国英 宋庆庆 王国华 潘相臣 刘冬冬 田普厚 石顺利 关平原 |
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| 作者单位： | 内蒙古农业大学兽医学院,内蒙古呼和浩特010018;金宇保灵生物药品有限公司兽用疫苗国家工程实验室,内蒙古 呼和浩特010030;内蒙古必威安泰生物科技有限公司,内蒙古呼和浩特011500;内蒙古通辽市家畜繁育指导站,内蒙古通辽028000 |
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| 摘 要： | 为促进布鲁菌病Rev.1疫苗及相关疫苗的研发, 该研究提取Rev.1疫苗株核酸, 应用PacBio平台进行全基因序列测定与分析。结果表明, Rev.1疫苗株基因组大小约3 299 187 bp, G+C含量为57.2%, 组装为染色体1、染色体2两条环状基因组, 大小分别为2 121 370、1 177 817 bp, G+C含量分别为57.2%、57.3%。将其Ery、BLS和VirB10基因序列与9株GenBank上发表的布鲁菌参考菌株的Ery、BLS和VirB10基因序列进行比较分析, 存在不同程度的差异, 同源性为97.4%~100%。
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| 关 键 词： | 布鲁菌 布鲁菌Rev.1疫苗株 全基因组测序 基因序列对比 |
| 收稿时间： | 2020-09-08 |
Genome-wide Sequencing and Analysis for Brucella Rev.1Vaccine Strain |
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| YU Huan-yu,ZHANG Zhi-dan,LIU Jian-qi,ZHAO Dan-tong,ZHAO Li-xia,LIU Guo-ying,SONG Qing-qing,WANG Guo-hua,PAN Xiang-chen,LIU Dong-dong,TIAN Pu-hou,SHI Shun-li,GUAN Ping-yuan.Genome-wide Sequencing and Analysis for Brucella Rev.1Vaccine Strain[J].Animal Husbandry and Feed Science,2020,41(5):91-95. |
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| Authors: | YU Huan-yu ZHANG Zhi-dan LIU Jian-qi ZHAO Dan-tong ZHAO Li-xia LIU Guo-ying SONG Qing-qing WANG Guo-hua PAN Xiang-chen LIU Dong-dong TIAN Pu-hou SHI Shun-li GUAN Ping-yuan |
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| Institution: | 1.College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;2.National Engineering Laboratory for Veterinary Vaccine, The Spirit Jinyu Biological Pharmaceutical Co., Ltd., Hohhot 010030, China;3.Inner Mongolia Bigvet Biotech Co., Ltd., Hohhot 011500, China;4.Livestock Breeding Guide Station of Tongliao City of Inner Mongolia, Tongliao 028000, China |
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| Abstract: | In order to promote the research and development of Brucella Rev.1 vaccine and related vaccines, the nucleic acid of the Brucella Rev.1 vaccine strain was extracted, and the genome-wide sequencing for Brucella Rev.1 vaccine strain was carried out and analyzed through PacBio platform. The results showed that the full-length genome of the Brucella Rev.1 vaccine strain was 3 299 187 bp, and the G+C content was 57.2%; two circular genomes of chromosome 1 and chromosome 2 were assembled; the size of chromosome 1 and chromosome 2 was 2 121 370 bp and 1 177 817 bp, respectively, and the G+C content of chromosome 1 and chromosome 2 was 57.2% and 57.3%, respectively. The sequences of Ery, BLS and VirB10 genes of the Brucella Rev.1 vaccine strain were bioinformatically compared and analyzed with their counterparts of 9 Brucella reference strains, and the sequence similarity among those genes were observed, ranging from 97.4% to 100%. |
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| Keywords: | Brucella Brucella Rev 1 vaccine strain genome-wide sequencing gene sequence comparison |
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