Schmallenberg virus challenge models in cattle: infectious serum or culture-grown virus? |
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| Authors: | Kerstin Wernike Michael Eschbaumer Angele Breithaupt Bernd Hoffmann Martin Beer |
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| Institution: | 1.Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), Suedufer 10, Greifswald, Insel Riems, 17493, Germany;2.Department of Experimental Animal Facilities and Biorisk Management, Friedrich-Loeffler-Institut (FLI), Suedufer 10, Greifswald, Insel Riems, 17493, Germany;3.Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada;4.Present address: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany |
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| Abstract: | Schmallenberg virus (SBV), discovered in Europe in 2011, causes mild transient disease in adult ruminants, but fetal infection can lead to severe malformation in cattle, sheep and goats.To elucidate the pathogenesis of this novel orthobunyavirus, considerable efforts are required. A reliable and standardized infection model is essential for in vivo studies. In the present study, two groups of four cattle were inoculated with either serum passaged in cattle only or cell culture-grown virus. The replication of culture-grown SBV in cattle was reduced compared to virus inoculated via infectious serum. In a second experiment, the infectious serum was titrated in calves; the tested batch contained 102.83 infectious doses per mL. Hence, serum-borne virus that was only passaged in the natural host is a suitable option for a standardized SBV infection model. |
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