| 福建省H9N2亚型禽流感病毒HA和NA基因的进化分析 |
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| 引用本文： | 朱春华,江斌,林甦,刘斌琼,陈珍,程龙飞,黄瑜.福建省H9N2亚型禽流感病毒HA和NA基因的进化分析[J].中国兽医寄生虫病,2011,19(5):32-38. |
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| 作者姓名： | 朱春华 江斌 林甦 刘斌琼 陈珍 程龙飞 黄瑜 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所,福州,350013 |
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| 基金项目： | 福建省重点项目，院青年创新基金 |
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| 摘 要： | 为进一步了解福建省H9N2亚型禽流感病毒的基因遗传进化关系,本研究将福建省2011年分离的毒株FZ-04、FZ-11与GenBank上登录的2000～2011年福建省分离的H9N2毒株及国内外典型代表株进行HA、NA基因的序列比对和遗传进化分析。结果表明,分离株FZ-04和FZ-11的HA基因与CK/FJ/G9/09株核苷酸同源性最高,属于国内常见的CK/BJ/1/94亚系。HA裂解位点处的氨基酸序列为-PSRSSR/GL-,符合低致病性禽流感病毒的分子特征。NA基因在遗传进化关系上呈现独立的分支,与CK/FJ/10954/05毒株核苷酸序列同源性最高,属于CK/HK/G9/97亚系,且NA基因推导的469个氨基酸序列中没有缺失。同时,从HA和NA基因的遗传进化树上可知,2000～2011年福建省H9N2禽流感病毒进化相对比较稳定,可能有一个共同的起源。
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| 关 键 词： | 禽流感病毒 H9N2亚型 HA基因 NA基因 进化分析 |
PHYLOGENETIC ANALYSIS OF HAEMAGGLUTININ AND NEURAMINIDASE GENES OF H9N2 INFLUENZA VIRUSES ISOLATEDFROM FUJIAN PROVINCE |
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| ZHU Chun-hua,JIANG Bin,LIN Su,LIU Bin-qiong,CHEN Zhen,CHENG Long-fei,HUANG Yu.PHYLOGENETIC ANALYSIS OF HAEMAGGLUTININ AND NEURAMINIDASE GENES OF H9N2 INFLUENZA VIRUSES ISOLATEDFROM FUJIAN PROVINCE[J].Chinese Journal of Veterinary Parasitology,2011,19(5):32-38. |
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| Authors: | ZHU Chun-hua JIANG Bin LIN Su LIU Bin-qiong CHEN Zhen CHENG Long-fei HUANG Yu |
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| Institution: | ZHU Chun-hua,JIANG Bin,LIN Su,LIU Bin-qiong,CHEN Zhen,CHENG Long-fei,HUANG Yu(Animal Husbandry and Veterinary Medicine Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China) |
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| Abstract: | Haemagglutinin（HA） and neuraminidase（NA） genes of two H9N2 subtype viruses（FZ-04 and FZ-11） isolated from different broiler flocks in Fujian in 2011 were amplified and sequenced in order to determine their genetic relationship with other Avian influenza virus strains that were either isolated from the same area during 2000-2011 or deposited in GenBank.The nucleotide sequences of haemagglutinin genes of both strains had the highest identity with CK/FJ/G9/09,indicating these strains belonged to the same major genetic CK/BJ/1/94 like sublineage.The FZ-04 and FZ-11 strains had-PSRSSR/GL-sequence at HA proteolytic cleavage sites,a characteristic of low pathogenic Avian influenza virus（LPAIV）.The neuraminidase gene of FZ-04 and FZ-11 strains encoded 469 amino acid sequences and had no nucleotides deletion.Phylogenetic analysis of NA genes showed that both strains had the highest homology with CK/FJ/10954/05 and belonged to genetic CK/HK/G9/97 like sublineage.On the basis of phylogenetic analysis of HA and NA genes,we concluded that H9N2 subtype Influenza viruses circulating in chicken flocks in Fujian during 2000-2011 might have a stable evolution and common origin. |
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| Keywords: | Avian influenza virus H9N2 subtype haemagglutinin neuraminidase phylogenetic analysis |
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