| Ⅰ型鸭肝炎病毒SH株全基因组序列测定与分析 |
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| 引用本文： | 宋翠萍,仇旭升,陈鸿军,于圣青,丁铲.Ⅰ型鸭肝炎病毒SH株全基因组序列测定与分析[J].中国兽医寄生虫病,2011,19(5):39-44. |
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| 作者姓名： | 宋翠萍 仇旭升 陈鸿军 于圣青 丁铲 |
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| 作者单位： | 中国农业科学院上海兽医研究所,上海,200241 |
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| 基金项目： | 公益性行业(农业)专项经费，中央级公益性科研院所基本科研业务专项经费 |
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| 摘 要： | 本研究从上海市某鸭场发病鸭群中分离到1株DHV-1型强毒株,对其进行毒价测定、琼脂扩散试验和动物回归实验,结果显示：按103.41 ELD50/0.2 mL接种1日龄雏鸭,该毒株对雏鸭有明显的致病性,死亡率为60%,肝脏病变率为100%。经全基因组测序显示：该毒株长度为7652 nt,含626 nt的5＇非编码区和314 nt的3＇非编码区,编码一个2249 aa大小的多聚蛋白。根据Blast比对发现,该毒株属于经典的DHV-1A群,与韩国经典强毒株R85952（99.7%）和中国分离强毒株HP-1（99.3%）的同源性最高。
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| 关 键 词： | 鸭病毒性肝炎Ⅰ型 分离 鉴定 全基因组序列 |
CHARACTERIZATION AND COMPLETE SEQUENCING OF A DUCK HEPATITIS VIRUS TYPE 1 ISOLATE FROM A DUCKLINGWITH ASCITES |
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| SONG Cui-ping,QIU Xu-sheng,CHEN Hong-jun,YU Sheng-qing,DING Chan.CHARACTERIZATION AND COMPLETE SEQUENCING OF A DUCK HEPATITIS VIRUS TYPE 1 ISOLATE FROM A DUCKLINGWITH ASCITES[J].Chinese Journal of Veterinary Parasitology,2011,19(5):39-44. |
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| Authors: | SONG Cui-ping QIU Xu-sheng CHEN Hong-jun YU Sheng-qing DING Chan |
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| Institution: | SONG Cui-ping,QIU Xu-sheng,CHEN Hong-jun,YU Sheng-qing,DING Chan(Shanghai Veterinary Research Institute,CAAS,Shanghai200241,China) |
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| Abstract: | A virus isolate SH obtained from a duckling with liver ascites was confirmed as duck hepatitis virus type 1（DHV-1） by determination of virus titer,immunological detection and animal experiments.The virus caused 60% mortality and 100% liver lesions when inoculated into one day-old ducklings with 103.41 ELD50/0.2 mL.The virus genome was 7652 nucleotides long（not containing the poly（A） tail） consisting of 5＇-and 3＇-terminal non-coding regions of 626 and 314 nucleotides.The single open reading frame encoded a polypeptide of 2249 amino acids.Comparative genome analysis with other available strains in GenBank indicated that SH strain shared 99.3%～99.7% similarity at the nucleotide level with R85952 and HP-1 strains that were also isolated in China. |
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| Keywords: | Duck hepatitis virus type 1 isolation characterization complete sequence |
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