| 地克珠利在雏鸡体内的药动学研究 |
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| 引用本文： | 张可煜,王国永,赵振升,索延乐.地克珠利在雏鸡体内的药动学研究[J].中国兽医寄生虫病,2008,16(2):1-4. |
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| 作者姓名： | 张可煜 王国永 赵振升 索延乐 |
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| 作者单位： | 1. 中国农业科学院上海兽医研究所,上海,200232
2. 河南科技大学动物科技学院,洛阳,471003 |
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| 基金项目： | 上海研发公共服务平台建设专项
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中国农业科学院上海兽医研究所青年科技基金 |
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| 摘 要： | 目的应用高效液相色谱测定单次给药后雏鸡体内地克珠利的血药浓度，研究其药动学规律。方法44羽雏鸡按10mg／kg．BW经口单次灌服地克珠利预混剂，采用高效液相色谱系统的流动相为乙腈：0．1％三氟乙酸：水，流速1．0mL／min，分流比57：20：23，固定相为TC-C18柱，检测波长280nm，测定地克珠利血浆浓度，计算其药动学参数。结果在0．3125～20μg／mL范围内，地克珠利血药浓度呈线性关系，最低检测浓度为0．16μg／mL，回收率在79．75％以上，日内RSD小于5．68％。单剂量给药后地克珠利的主要药动学参数为：血药浓度峰值（Cmax）32．97μg／mL，达峰时间（Tpeak）1．64h，消除半衰期（T1／2β）24．29h，药时曲线下面积（AUC）349．47（mg／L）·h，血浆清除率（CL）0．03mg／kg·h。结论地克珠利在雏鸡体内代谢符合一级吸收的二室模型，药物吸收比较快和消除缓慢。
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| 关 键 词： | 地克珠利 雏鸡 药动学 高效液相色谱 |
| 文章编号： | 1005-0868(2008)02-0001-04 |
| 修稿时间： | 2007年12月19 |
PHARMACOKINETICS STUDY OF DICLAZURIL IN CHICKEN |
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| ZHANG Ke-yu,WANG Guo-yong,ZHAO Zhen-sheng,SUO Yan-le.PHARMACOKINETICS STUDY OF DICLAZURIL IN CHICKEN[J].Chinese Journal of Veterinary Parasitology,2008,16(2):1-4. |
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| Authors: | ZHANG Ke-yu WANG Guo-yong ZHAO Zhen-sheng SUO Yan-le |
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| Institution: | ZHANG Ke-yu , WANG Guo-yong, ZHAO Zhen-sheng, SUO Yan-le ( 1. Shanghai Veterinary Research Institute of CAAS, Shanghai 200232, China ; 2. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China) |
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| Abstract: | Objective To determine the concentrations of diclazuril in chicken plasma after single oral gavage administration by HPLC method and to evaluate the pharmacokinetics of diclazuril. Methods The drug concentration of 44 chickens plasma were determined by HPLC method following single oral administration at the dosage of 10 mg/kg. BW. A TC-C18 column and the 280 nm wavelength of ultraviolet were used to separate and detect diclazuril with a mobile phase of a mixture of acetonitrile ： 0.1% trifluoroacetic acid ： water, at a flow rate of 1.0 mL/min. The data of plasma concentrations were used to caculate the pharmacokinetics of the drug. Results The linear range of the standard curve of diclazuril was 0.312 5 - 20 μg/mL, and the determintation limit was 0.16 μg/mL. The extraction recoverys were more than 79.75%, inter-day RSDs were lass than 5.68%. After a single dose, the main pharmacokinetics parameters were as follows ： Tpeak （ 1.64 ） h, Cmax （ 32.97 ） μg/mL, T1/2β （24.29） h, AUC0-1 （349.47） （mg/L）·h, CL （0.03） mg/kg·h. Conclusion The process of diclazuril in chickens was best fitted a two-compartmental with one order absorption model, and the drug was absorbed fast and eliminated slowly after single p. o. administration. |
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| Keywords: | Diclazufil chicken pharmacokinetics HPLC |
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