| HPLC法测定清瘟败毒颗粒中盐酸小檗碱的含量研究 |
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| 引用本文： | 郭时金,王艳萍,周春凤,董林,徐倩倩,张志美,沈志强.HPLC法测定清瘟败毒颗粒中盐酸小檗碱的含量研究[J].家畜生态学报,2013,34(4):37-40. |
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| 作者姓名： | 郭时金 王艳萍 周春凤 董林 徐倩倩 张志美 沈志强 |
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| 作者单位： | 1.山东省滨州畜牧兽医研究院，山东 滨州 256600；2.山东绿都安特动物药业有限公司，山东 滨州 256600 |
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| 基金项目： | 山东省技术创新项目（201210916001） |
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| 摘 要： | 为了建立"清瘟败毒颗粒"中盐酸小檗碱含量的高效液相色谱检测方法(high-per-formance liquid chromatography,HPLC),采用SB-C18柱(StableBond,4.6×150 mm,5μm),流动相:乙腈-0.05mol/L磷酸二氢钾溶液(50∶50),紫外检测波长为345nm,流速1.0mL/min,柱温30℃。在选择色谱条件下,盐酸小檗碱与辅料、溶剂及清瘟败毒颗粒中的其他成分的峰之间分离良好,在16.0～48.0μg/mL范围内峰面积与盐酸小檗碱含量直线关系(r2=1,n=6),平均回收率为99.09%。该方法简便、灵敏、可重复性好,可用于清瘟败毒颗粒的质量控制。
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| 关 键 词： | 盐酸小檗碱 高效液相色谱法 清瘟败毒颗粒 |
Determination of Berberine Hydrochloride in Qingwen Baidu Granule by HPLC |
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| GUO Shi-jin,WANG Yan-ping,ZHOU Chun-feng,DONG Lin,XU Qian-qian,ZHANG Zhi-mei,SHEN Zhi-qiang.Determination of Berberine Hydrochloride in Qingwen Baidu Granule by HPLC[J].Acta Ecologae Animalis Domastici,2013,34(4):37-40. |
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| Authors: | GUO Shi-jin WANG Yan-ping ZHOU Chun-feng DONG Lin XU Qian-qian ZHANG Zhi-mei SHEN Zhi-qiang |
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| Institution: | 1.Binzhou Academy of Animal Science and Veterinary Medicine,Binzhou, Shandong 256600, China;
2. Shandong Lvdu Ante Veterinary Drug Co., Ltd. ,Binzhou, Shandong 256600, China |
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| Abstract: | To establish a simple and sensitive HPLC method for determining berberine hydrochloride in Qingwen Baidu granule,HPLC method was used. The HPLC system consisted of Stable Bond C18 column(4.6×150 mm, 5 μm) and a mobile phase of acetonitrile 0.05mol/L potassium dihydrogen phosphate solution (50∶50, v∶v) mixture. Berberine hydrochloride was detected at 345 nm, with a flow rate of 1.0 mL/min. The column temperature was maintained at 30 ℃. The excipients and solvents in the granule could be well separated from the drug under such a designated chromatogram condition and did not interfere with the assay. A good linear relationship was found between peak area and the concentration of berberine hydrochloride in the range of 16.0~48.0 μg/mL. The average recovery of berberine Hydrochloride was 99.09%. The established approach was specific, accurate, reliable, prompt, sensitive and applicable. It could be used to control the quality of Qingwen Baidu granule |
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| Keywords: | berberine hydrochloride HPLC Qingwen Baidu granule |
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