Allele distributions of two novel SNPs within the sheep Cyp19 gene |
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| Authors: | Attila Zsolnai István Anton László Fésüs one Estonba Manfred Schwerin Jens Vanselow |
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| Institution: | Research Institute for Animal Breeding and Genetics, Gesztenyés, Herceghalom, Hungary,;Department of Animal Biology and Genetics, University of the Basque Country UPV/EHU, Bilbao, Spain, and;Research Unit Molecular Biology, Research Institute for the Biology of Farm Animals, Wilhelm-Stahl-Allee Dummerstorf, Germany |
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| Abstract: | Oestrogen is an important regulator of reproduction and growth. The key enzyme of oestrogen biosynthesis, aromatase cytochrome P450, is encoded by the Cyp19 gene. In order to generate genetic markers for the sheep Cyp19 gene, two novel single nucleotide polymorphisms (SNPs), one located in promoter 2 (P2), the other one in intron 9 (I9), were identified by a comparative sequencing approach. The allele distributions of both SNPs were investigated by means of Polymerase chain reaction‐restriction fragment length polymorphism analysis (PCR‐RFLP) in five economically relevant sheep breeds (British Milk Sheep, Carranzana, Latxa Black Face, Latxa White Face, Merino) and three ancient Hungarian breeds kept as gene reserves (Cikta, Racka, Tsigai). In British Milk Sheep, only the intronic SNP was present whereas in Merino, Cikta, Racka, Tsigai, Carranzana, Latxa Black Face and Latxa White Face, both SNPs could be found. This indicates that the newly identified SNPs can be used as markers for the Cyp19 locus in various sheep breeds. |
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