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| 高效液相色谱法研究牛乳源耐药金黄色葡萄球菌对环丙沙星的蓄积作用 | |
| 引用本文： | 曹兴元,白如念,罗应武,吴聪明.高效液相色谱法研究牛乳源耐药金黄色葡萄球菌对环丙沙星的蓄积作用[J].中国兽医杂志,2011,47(8). |
| 作者姓名： | 曹兴元 白如念 罗应武 吴聪明 |
| 作者单位： | 1. 中国农业大学动物医学院,北京海淀,100193 2. 北京市畜牧兽医总站,北京朝阳100185; 3. 北京市怀柔区动物疫病预防控制中心,北京怀柔,101400 |
| 基金项目： | 国家自然科学基金(30700604) |
| 摘 要： | 近年来研究发现,金黄色葡萄球菌的NorA、NorB和NorC外排泵蛋白介导对氟喹诺酮类药物的耐药.本文采用临床分离的牛乳源环丙沙星耐药的金黄色葡萄球菌,通过建立的高效液相色谱法(HPLC-FLD),测定了临床分离的耐药、敏感菌及金黄色葡萄球菌标准菌株ATCC 29213对环丙沙星积聚量的差异,为临床检测通过外排泵介导的氟喹诺酮类药物耐药的金黄色葡萄球菌提供了方法学上的依据. |
| 关 键 词： | 环丙沙星 金黄色葡萄球菌 高效液相色谱 蓄积 |
Accumulation effect of ciprofloxacin in Staphylococcus aureus isolated from milk by HPLC |
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| CAO Xing-yuan,BAI Ru-nian,LUO Ying-wu,WU Cong-ming.Accumulation effect of ciprofloxacin in Staphylococcus aureus isolated from milk by HPLC[J].Chinese Journal of Veterinary Medicine,2011,47(8). | |
| Authors: | CAO Xing-yuan BAI Ru-nian LUO Ying-wu WU Cong-ming |
| Institution: | CAO Xing-yuan1,BAI Ru-nian2,LUO Ying-wu3,WU Cong-ming1(1.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China,2.Animal Husbandry and Veterinary Station,Beijing 100185,3.Center for Animal Disease Control of HuaiRou District,Beijing 101400,China) |
| Abstract: | In recent years,it has been found that NorA,NorB and NorC efflux pump protein result in resistance of fluoroquinolone in Staphylococcus aureus.In this paper,accumulation effect of ciprofloxacin in clinical resistant isolates,sensitive strains and ATCC 29213 standard Staphylococcus aureus was assayed with or without CCCP by the High Performance Liquid Chromatography(HPLC-FLD).The established HPLC method can be used to screen out Staphylococcus aureus strains resistant to fluoroquinolone through efflux pump. |
| Keywords: | Ciprofloxacin Staphylococcus aureus high performance liquid chromatography accumulation |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |