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| 细菌16SrDNA基因芯片的构建及应用 | |
| 引用本文： | 林居纯,曹三杰,蒋红霞,曾振灵.细菌16SrDNA基因芯片的构建及应用[J].中国兽医杂志,2011,47(4). |
| 作者姓名： | 林居纯 曹三杰 蒋红霞 曾振灵 |
| 作者单位： | 1. 华南农业大学兽医学院广东省兽药研制与安全评价重点实验室,广东广州510642;四川农业大学动物医学院,四川雅安625014 2. 四川农业大学动物医学院,四川雅安,625014 3. 华南农业大学兽医学院广东省兽药研制与安全评价重点实验室,广东广州,510642 |
| 基金项目： | 国家自然科学基金，四川省优势产业发展急需紧缺专业博士后项目 |
| 摘 要： | 本试验为建立能检测兽医临床重要病原菌的基因芯片方法,采用通用引物扩增菌株16S rDNA V1-V3区,制备16SrDNA PCR产物基因芯片,对5种兽医临床微生物进行检测.结果显示,制备的基因芯片能特异性地检测金黄色葡萄球菌、链球菌和鸡毒支原体,以及这3种菌株混合样品,但对大肠杆菌及沙门菌检测结果不理想.基因芯片检测灵敏度为3μg/L. |
| 关 键 词： | 基因芯片 病原菌 16S rDNA 检测 |
Development and application of 16S rDNA microarray for the detection of clinical bacteria |
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| LIN Ju-chun,CAO San-jie,JIANG Hong-xia,ZENG Zhen-ling.Development and application of 16S rDNA microarray for the detection of clinical bacteria[J].Chinese Journal of Veterinary Medicine,2011,47(4). | |
| Authors: | LIN Ju-chun CAO San-jie JIANG Hong-xia ZENG Zhen-ling |
| Institution: | LIN Ju-chun 1,2,CAO San-jie2,JIANG Hong-xia1,ZENG Zhen-ling1(1.College of Veterinary Medicine,South China Agricultural University,Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation,Guangzhou 510642,China,2.College of Veterinary Medicine,Sichuan Agricultural University,Yaan 625014,China) |
| Abstract: | DNA microarray method was developed for detection of veterinary important pathogenic bacteria.The DNA of bacteria was amplified by universal primers targeted to16S rDNA V1-V3 domain,and 16S rDNA clone probes were arrayed on microarray slides,then the constructed 16S rDNA microarray was used to detect 5 different microorganisms.The hybridization results showed that S.aureus,S.agalactiae and M.gallisepticum could be identified correctly by 16S rDNA microarray,The mixed samples of them could also be discrimina... |
| Keywords: | microarray pathogenic bacteria 16S rDNA detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |