| 产α-半乳糖苷酶菌株的筛选、鉴定及其酶学特性的研究 |
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| 引用本文： | 董岩岩,张燕婕,黄遵锡,周峻沛,唐湘华,李俊俊.产α-半乳糖苷酶菌株的筛选、鉴定及其酶学特性的研究[J].饲料工业,2011,32(14). |
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| 作者姓名： | 董岩岩 张燕婕 黄遵锡 周峻沛 唐湘华 李俊俊 |
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| 作者单位： | 云南师范大学生命科学学院,650092,云南昆明 |
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| 基金项目： | 国家“863”项目[0120052211020] |
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| 摘 要： | 利用筛选培养基从不同豆制品作坊附近的土样中筛选出一株产α-半乳糖苷酶的菌株D-1,对其进行分子鉴定及所产的α-半乳糖苷酶进行酶学特性研究。研究表明,菌株D-1为Aspergillus niger,此菌株所产α-半乳糖苷酶的最适反应温度是55℃,在60℃以下热稳定性较好;最适反应pH值为5.0,在pH值3.0～5.5范围内稳定性较好,相对酶活>64.1%;Mg2+、Na+、Pb2+、K+、Mn2+、Co2+、Al3+对α-半乳糖苷酶均有不同程度的抑制作用,其中Cu2+和Fe3+的抑制作用较为明显,而Ca2+、EDTA(乙二胺四乙酸)、Zn2+对α-半乳糖苷酶有一定的促进作用。
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| 关 键 词： | α-半乳糖苷酶 筛选 分子鉴定 酶学特性 |
Screening, identification and research of enzymatic characterization of a strain producing α-galactosides |
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| Dong Yanyan,Zhang Yanjie,Huang Zunxi,Zhou Junpei,Tang Xianghua,Li Junjun.Screening, identification and research of enzymatic characterization of a strain producing α-galactosides[J].Feed Industry,2011,32(14). |
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| Authors: | Dong Yanyan Zhang Yanjie Huang Zunxi Zhou Junpei Tang Xianghua Li Junjun |
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| Institution: | Dong Yanyan,Zhang Yanjie,Huang Zunxi,Zhou Junpei,Tang Xianghua,Li Junjun |
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| Abstract: | By using a selective medium,strain D-1was screened from the different bean product workshop.This strain was capable to produce the maximal quantity of α-galactosidase among all our screened strains.The enzymatic properties of the enzyme and molecular identification were studied.The strain is Aspergillus niger.It showed that the optimal pH and temperature was 5.0 and 55 ℃ respectively;the enzyme activity was stable below 60 ℃ within pH of 3.0 to 5.5,and the residual enzymatic activity was above 64.1%.However,its activity was inhibited by Mg2+,Na+,Pb2+,K+,Mn2+,Co2+,Al3+ and was strongly inhibited by Cu2+,Fe3+ and moderately activated by Ca2+,EDTA and Zn2+. |
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| Keywords: | α-galactosidase screening molecular identification enzymatic characterization |
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