| 一株产耐热纤维素酶放线菌的固体发酵研究 |
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| 引用本文： | 邵继海,胡俊林,孙毓临,何绍江,冯新梅.一株产耐热纤维素酶放线菌的固体发酵研究[J].饲料工业,2006,27(24):37-39. |
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| 作者姓名： | 邵继海 胡俊林 孙毓临 何绍江 冯新梅 |
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| 作者单位： | 1. 湖南农业大学资源环境学院,410128,湖南,长沙
2. 华中农业大学农业微生物国家重点实验室 |
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| 摘 要： | 以一株产耐热纤维素酶放线菌为材料,研究了该菌固体发酵培养基组分的改良及固体发酵工艺的优化,同时探讨了该菌与酵母混菌发酵时的产酶情况。实验结果表明,该菌株的最适培养基成分为:稻草粉与麸皮比为1、豆粕30%、KH2PO41%、MgSO4·7H2O0.2%。最佳的发酵工艺条件为:培养基的初始pH值为7、发酵温度为35℃、培养基固与水体积比为0.8、菌株接种量为5%。该菌固体产酶发酵过程中第72h接入酵母菌的效果较好,酵母菌最佳接种量为1%,浓度为4.6×105cfu/ml。
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| 关 键 词： | 纤维素放线菌 固体发酵 酵母菌 |
| 收稿时间： | 2006-10-19 |
| 修稿时间： | 2006年10月19 |
Study of solid-state fermentation of an actinomycete producing thermophilic cellulase |
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| Shao Jihai,Hu Junlin,Sun Yulin,He Shaojiang,Feng Xinmei.Study of solid-state fermentation of an actinomycete producing thermophilic cellulase[J].Feed Industry,2006,27(24):37-39. |
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| Authors: | Shao Jihai Hu Junlin Sun Yulin He Shaojiang Feng Xinmei |
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| Abstract: | We studied the optimum cultural medium to solid-state fermentation of an actinomycete producing thermophilic cellulose and the optimum cultural conditions for producing cellulase.The result of our experiments showed that the optimum culture medium for solid-state fermentation as follows:ratio of straw to bran is 1,bean cake 30%,KH2PO4 1%,MgSO4·7H2O 0.2%.The optimum cultural conditions for producing cellulase were as follows:the initial pH of the culture medium is 6,the fermentation temperature is 35 ℃,the ratio of dry weigh of culture medium to water is 0.8,the inoculation quantity is 5%.The best time for inoculating saccharomyces cerevisiae was the 72 h after the beginning of fermentation,and the optimum quantity for inoculating was 1% with the concentration of 4.6×105 cfu/ml. |
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| Keywords: | cellulose-decomposing strain solid-state fermentation saccharomyces cerevisiae |
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