| 饲用酸化剂中甲酸、乙酸、丙酸、乳酸、柠檬酸的同步测定 |
| |
| 引用本文： | 索德成,李兰,樊霞.饲用酸化剂中甲酸、乙酸、丙酸、乳酸、柠檬酸的同步测定[J].饲料工业,2012,33(21). |
| |
| 作者姓名： | 索德成 李兰 樊霞 |
| |
| 作者单位： | 中国农业科学院农业质量标准与检测技术研究所,北京,100081 |
| |
| 摘 要： | 实验建立利用高效液相色谱同时测定饲用酸化剂中甲酸、乙酸、丙酸、乳酸、柠檬酸含量的方法。采用Grom-Sil org acid柱(250 mm×2.1 mm,5μm)进行分析。流动相为pH值2.5磷酸水溶液;流速为1.0 ml/min;检测波长为210 nm;柱温为30℃。在此色谱条件下,5种有机酸分离良好。平均回收率均大于85%,RSD小于10.0%。对实际样品的检测结果表明:该方法操作简便易行,准确可靠。
|
| 关 键 词： | 饲用酸化剂 含量测定 高效液相色谱法 |
Determination of formic acid, acetic acid, propionic acid, lactic acid, citric acid in feed acidifier |
| |
| Suo Decheng
,
Li Lan
,
Fan Xia.Determination of formic acid, acetic acid, propionic acid, lactic acid, citric acid in feed acidifier[J].Feed Industry,2012,33(21). |
| |
| Authors: | Suo Decheng Li Lan Fan Xia |
| |
| Abstract: | A method of the simultaneous determination of the contents of formic acid,acetic acid,propionic acid,lactic acid,citric acid in feed acidifier by HPLC was established.The separation was performed on Grom-Silorg acid(250 mm×4.6 mm,5 μm) with the mobile phase of hydrochloric acid solution(pH 2.5).The detection wave length was 210 nm.The flow rate was 1.0 ml/min and the column temperature was 30 ℃.Good linearities of five acid were showed.The average recoveries were greater than 85% respectively,RSD was less than 10.0%.The result of real sample have been shown:the method was simple,rapid and reliable for the simultaneous determination of formic acid,acetic acid,propionic acid,lactic acid,citric acid in organic acids. |
| |
| Keywords: | feed acidifier determination HPLC |
| 本文献已被 万方数据 等数据库收录！ |
|
