| 中国鸭源H5N6 AIV血凝素和神经氨酸酶基因特征及遗传进化分析 |
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| 引用本文： | 陈翠腾,万春和,陈珍,朱春华,傅光华,施少华,蔡国漳,刘斌琼,黄瑜.中国鸭源H5N6 AIV血凝素和神经氨酸酶基因特征及遗传进化分析[J].中国家禽,2020(1):21-27. |
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| 作者姓名： | 陈翠腾 万春和 陈珍 朱春华 傅光华 施少华 蔡国漳 刘斌琼 黄瑜 |
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| 作者单位： | 福建省农业科学院畜牧兽医研究所 |
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| 基金项目： | 福建省属公益类科研院所基本科研专项(2017R1023-6、2019R1026-4);现代农业产业体系建设专项资金(CARS-42);福建省自然科学基金项目(2017J01059);福建省财政专项(FJFS-2018)。 |
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| 摘 要： | 为明确中国鸭源H5N6 AIV的HA和NA基因特征及其遗传变异情况,研究选取了NCBI流感病毒资源库里的32株具有完整ORF编码区的中国鸭源H5N6 AIV的HA和NA基因序列,利用分子生物学软件分析其HA和NA基因特征及其遗传进化情况。结果显示,中国鸭源H5N6 AIV的HA裂解位点有REKRRKR↓G、RERRRKR↓G和KEKRRKR↓G三种类型,HA有6个潜在的N-糖基化位点,其中第182、222、224位氨基酸依次为N、Q、G,具有与禽源唾液酸α-2,3受体结合的特性,然而HA发生了S123P/T、T156A、S223R氨基酸位点突变,部分毒株发生了I151T氨基酸位点突变;HA基因核苷酸遗传进化分析显示,中国鸭源H5N6 AIV遗传进化上均处于clade2.3.4.4分支,并进一步进化出Ⅰ和Ⅱ两个亚分支。部分中国鸭源H5N6 AIV NA第59~69位缺失11个氨基酸,中国鸭源H5N6 AIV NA基因遗传进化上分为两个大的分支,分别对应NA基因颈部缺失型和完整型两种类型;NA颈部完整型的NA有6个潜在的N-糖基化位点,NA颈部缺失型的NA由于第59~69位氨基酸的缺失,导致其第67位丢失了一个N-糖基化位点。研究为中国鸭源H5N6 AIV的生物学特性和遗传进化特征研究奠定基础。
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| 关 键 词： | 鸭 H5N6亚型禽流感病毒 血凝素 神经氨酸酶 基因特征 遗传进化 |
Gene Characteristics and Genetic Evolution Analysis of Hemagglutinin and Neuraminidase of H5N6 Subtype Avian Influenza Virus from Duck in China |
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| CHEN Cuiteng,WAN Chunhe,CHEN Zhen,ZHU Chunhua,FU Guanghua,SHI Shaohua,CAI Guozhang,LIU Binqiong,HUANG Yu.Gene Characteristics and Genetic Evolution Analysis of Hemagglutinin and Neuraminidase of H5N6 Subtype Avian Influenza Virus from Duck in China[J].China Poultry,2020(1):21-27. |
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| Authors: | CHEN Cuiteng WAN Chunhe CHEN Zhen ZHU Chunhua FU Guanghua SHI Shaohua CAI Guozhang LIU Binqiong HUANG Yu |
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| Institution: | (Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention,Fujian Animal Diseases Control Technology Development Center,Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013) |
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| Abstract: | In order to clarify the HA and NA gene characteristics and genetic variation of H5 N6 subtype AIV from duck in China, the HA and NA gene sequences with complete ORF coding region of 32 H5 N6 subtype AIV from duck in China were selected from the NCBI influenza virus database, and molecular biology software was used to analyze the characteristics of HA and NA genes and their genetic evolution. The results showed that the HA cleavage sites of H5 N6 subtype AIV from duck in China have three types: REKRRKR↓G, RERRRKR↓G and KEKRRKR↓G. HA has six potential N-glycosylation sites. The amino acids at positions 182, 222 and 224 of HA are N, Q and G, which have the characteristics of binding to avian sialic acid α-2, 3 receptors. However, amino acid sites mutation of S123 P/T,T156 A and S223 R occur in HA, and I151 T amino acid mutation occurred in some strains. Nucleotide genetic evolution analysis of HA gene showed that H5 N6 subtype AIV from duck in China was genetically evolved in the branch of clade2.3.4.4, and further developed two sub-branches of Ⅰ and Ⅱ. Some H5 N6 subtype AIV from duck in China lack 11 amino acids at positions 59 to 69 of NA. The nucleotide genetic evolution analysis of NA gene showed that the NA gene of H5 N6 subtype AIV from duck in China was genetically evolved into two large branches, corresponding to the NA gene neck loss type and intact type. The neck intact type NA gene have six potential N-glycosylation sites.The neck loss type NA lost an N-glycosylation site at position 67 due to deletion of amino acids 59 to 69. This study lays a foundation for the study of the biological characteristics and genetic evolution characteristics of H5 N6 subtype AIV from duck in China. |
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| Keywords: | duck H5N6 AIV hemagglutinin neuraminidase gene characteristics genetic evolution |
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