| 藏鸡NSTN基因克隆与mRNA组织表达谱分析 |
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| 引用本文： | 毛亮,徐亚欧,杨孔.藏鸡NSTN基因克隆与mRNA组织表达谱分析[J].中国家禽,2011,33(16). |
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| 作者姓名： | 毛亮 徐亚欧 杨孔 |
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| 作者单位： | 1. 泸州医学院心肌电生理研究室,四川泸州,646000
2. 西南民族大学生命科学与技术学院,四川成都,610041 |
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| 基金项目： | 四川省青年科技基金(08ZQ026-027); 西南民族大学中央高校基础科研基金(09NZYZJ03) |
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| 摘 要： | 肌肉生长抑制素(MSTN)是骨骼肌发育的负性调控因子,本研究用RT-PCR方法克隆藏鸡MSTN编码基因,用半定量RT-PCR方法检测MSTN mRNA在藏鸡腿肌等10个组织的表达情况.结果显示,藏鸡MSTN DNA长1128 bp,编码375个氨基酸.藏鸡与原鸡氨基酸序列一致率为99.7％,有6个同义突变和1个非同义突变的氨基酸差异.MSTN mRNA在腿肌、胸肌、心脏、肾脏、睾丸、卵巢、胃中均检测到表达,脂肪、肝脏、肺中未检测到MSTN mRNA表达.MSTN mRNA表达水平由高到低的顺序为:腿肌、胸肌、心脏、肾脏、睾丸、卵巢和胃.
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| 关 键 词： | 藏鸡 肌肉生长抑制素 克隆 半定量RT-PCR |
Molecular Cloning and Tissue-specific Expression Profile of Myostatin (MSTN) Gene of Tibetan Gallus |
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| MAO Liang,XU Ya'ou,YANG Kong.Molecular Cloning and Tissue-specific Expression Profile of Myostatin (MSTN) Gene of Tibetan Gallus[J].China Poultry,2011,33(16). |
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| Authors: | MAO Liang XU Ya'ou YANG Kong |
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| Abstract: | Myostatin (MSTN) is a negative regulatory factor of skeletal muscle growth. In this paper,Tibetan Gallus MSTN coding gene was cloned by RT-PCR. At the same time,differential expressions were examined with semi-quantitative RT-PCR among leg muscle,pectoral muscle,fat,heart,liver,lung,stomach,kidney,testis and ovary of Tibetan Gallus. The MSTN DNA contained 1 128 bp and coded 375 amino acids. The sequence shared 99.7% homology with Gallus gallus,and 6 synonymous and 1 non-synonymous mutation in amino acids. M... |
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| Keywords: | Tibetan Gallus MSTN cloning semi-quantitative RT-PCR |
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