| 应用PCR快速检测鸭疫里默氏杆菌的研究 |
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| 引用本文： | 李春芬,李郁,魏建忠,张小飞,黄显明.应用PCR快速检测鸭疫里默氏杆菌的研究[J].中国家禽,2008,30(1):18-20. |
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| 作者姓名： | 李春芬 李郁 魏建忠 张小飞 黄显明 |
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| 作者单位： | 1. 安徽农业大学动物科技学院,安徽合肥,230036
2. 江苏省农科院兽医研究所,江苏南京,210014;南京天邦生物科技有限公司,江苏南京,211102 |
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| 摘 要： | 根据鸭疫里默氏杆菌（RA）16SrDNA基因序列设计引物，对1株RA阳性株、9株临床分离鉴定的RA、3株大肠杆菌、1株多杀性巴氏杆菌和1株沙门氏菌进行PCR扩增，结果所有RA均出现643bp特异性扩增条带，其余非RA则均未扩增出特异性条带，表明该对引物及建立的PCR具有很强的特异性。优化的PCR反应体系能检出RA的最低DNA量为20pg。菌落直接PCR是增强RA快速检测鉴定的手段。应用PCR对病料组织直接进行RA检测，脑组织为首选检测对象，具有良好的实际应用意义。
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| 关 键 词： | 鸭疫里默氏杆菌RA PCR 检测 快速 应用意义 快速检测 鸭疫里默氏杆菌 研究 Detection 对象 脑组织 手段 检测鉴定 增强 菌落直接 反应体系 优化 特异性条带 扩增条带 结果 沙门氏菌 多杀性巴氏杆菌 大肠杆菌 分离鉴定 |
| 收稿时间： | 2007-08-14 |
| 修稿时间： | 2007-10-25 |
Quick Detection of Riemerella anatipestifer by PCR |
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| LI Chunfen,LI Yu,WEI Jianzhong,ZHANG Xiaofei,HUANG Xianming.Quick Detection of Riemerella anatipestifer by PCR[J].China Poultry,2008,30(1):18-20. |
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| Authors: | LI Chunfen LI Yu WEI Jianzhong ZHANG Xiaofei HUANG Xianming |
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| Abstract: | According to 16 S rDNA gene sequence from Riemerella anatipestifer,a pair of primer was designed and synthesized. PCR was used to detect one positive strain of RA,9 isolates of RA,3 strains of Escherichia coli, 1 strain of Pasteurella multocida and 1 strain of Salmonella gallinarum. The results showed that a 643 bp DNA fragment was expected ampilify from all detected RA strains while others were negative, which confirmed that the primer and PCR had the high specificity. The lowest amount of detectable DNA of RA by PCR was 20 pg. RA could be detected more quickly and exactly by PCR from bacteria. The brain tissues was the first material when application of PCR to detect RA from the pathological tissues. This methods could make good use in practice. |
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| Keywords: | Riemerella anatipestifer PCR detection rapid |
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