| 抗褐化剂对荻外植体褐化和愈伤组织生长的影响 |
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| 引用本文： | 彭思佳,丁力,刘清波,陈智勇,肖亮,易自力,蒋建雄.抗褐化剂对荻外植体褐化和愈伤组织生长的影响[J].草原与草坪,2015(5). |
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| 作者姓名： | 彭思佳 丁力 刘清波 陈智勇 肖亮 易自力 蒋建雄 |
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| 作者单位： | 1. 湖南农业大学 生物科学技术学院芒属植物研究所,湖南 长沙,410128;2. 湖南农业大学 生物科学技术学院芒属植物研究所，湖南 长沙 410128; 江苏大学 生物质能源研究所，江苏 镇江 212013 |
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| 摘 要： | 以荻(Miscanthus sacchariflorus)幼穗和诱导出的愈伤组织为材料探讨抗褐化剂聚乙烯吡咯烷酮(PVP)、抗坏血酸(Vc)、柠檬酸(CA)对外植体和愈伤组织抗褐化作用,以及愈伤诱导率及愈伤生长速度的影响。结果表明,3种抗褐化剂都能抑制外植体和愈伤组织褐化,并提高愈伤组织诱导率。添加0.2g/L PVP时荻外植体的褐变程度最低,0.2g/L CA时愈伤组织诱导率最高,为91.1%,向愈伤组织继代培养基中添加0.2g/L的Vc愈伤组织重量增加倍数最高,为21.38倍,且愈伤组织褐化率低为25.8%。
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| 关 键 词： | 荻 抗褐化剂 褐化 愈伤生长 |
Effect of antioxidant on explant browning and callus growth of Miscanthus sacchariflorus |
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| Abstract: | The effects of three antioxidants,including polyvinyl pyrrolidone (PVP),ascorbic acid (Vc)and citric acid (CA)on browning of explants of immature inflorescences from Miscanthus sacchariflorus and their induced calli,callus induction and callus growth were studied.The results suggested that all of these three an-tioxidants could reduce the explant and callus browning and increase the callus induction rate.PVP had the best effect on anti-browning of the explants.By adding 0.2 g/L concentration of CA,the callus induction rate could reach the highest value (91.1 %).Supplementation with 0.2 g/L concentration of Vc in the callus subculture medium could increase the callus weight by 21.38 times and reduce the callus browning rate by 25.8%. |
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| Keywords: | Miscanthus sacchariflorus antioxidant browning callus growth |
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