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燕麦散黑穗病菌冬孢子萌发条件研究
引用本文:张玉霞,赵桂琴,姚 拓,等.燕麦散黑穗病菌冬孢子萌发条件研究[J].草原与草坪,2014(2):33-38.
作者姓名:张玉霞  赵桂琴  姚 拓  
作者单位:甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续发展研究中心,甘肃兰州730070
基金项目:现代农业产业技术体系(CARS-08-C),农业行业专项(201003023)和甘肃省农业科技创新项目(GNCX-2012-45)资助
摘    要:为明确燕麦散黑穗病菌(Ustilago avenae)冬孢子在自然生境中的萌发条件,研究了疏亲水界面、不同温度、湿度、光照时间、pH、碳源和氮源对冬孢子萌发的影响。结果表明,亲水界面促进冬孢子萌发,在水琼脂培养基上萌发率最高;冬孢子萌发的最适温度为20~25℃,温度过高或过低均抑制其萌发。冬孢子对湿度要求较高,RH≥90%时才可萌发,且萌发率较低。光照对冬孢子萌发的影响不明显,酸性条件有利于冬孢子萌发,最适pH为4~7,葡萄糖和蛋白胨可促进冬孢子萌发。

关 键 词:燕麦散黑穗病菌  冬孢子  萌发条件

Study on teliospore germination conditions of Ustilago avenae in oats
ZHANG Yu-xia,ZHAO Gui-qin,YAO Tuo,WANG Guo-ji,MA Wen-wen.Study on teliospore germination conditions of Ustilago avenae in oats[J].Grassland and Turf,2014(2):33-38.
Authors:ZHANG Yu-xia  ZHAO Gui-qin  YAO Tuo  WANG Guo-ji  MA Wen-wen
Institution:(College of Pratacultural Science ,Gansu Agricultural University/Key Laboratory of Grassland Ministry of Education/Pratacultural Engineering Laborat for Grazingland Ecosystem Sustainability ory of Gansu Province/Sino-U. S. ,Lanzhou 730070, China) Ecosystem, Centers
Abstract:In the paper,we studied the effects of lyophobic and hydrophilic interface,different temperature, humidity,light illumination time,pH,carbon sources and nitrogen sources on Ustilago avenae teliospore germination.The results showed that the hydrophilic interface could promote Ustilago avenae teliospore germination, The germination rate was highest on water agar medium;The optimum temperature of teliospore germination was from 20 to 25 ℃,Teliospore germination was inhibited by too high or too low on the temperature;The demand of humidity for teliospore was high,they could only germinate when the relative humidity was higher than 90%,and germination rate was low;The illumination time had not obvious effect on teliospore germination;A-cidic conditions could promote teliospore germination,the optimum pH was 4 to 7;Glucose and peptone could promote the teliospore germination.
Keywords:Ustilago avenae  teliospore  germination conditions
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