| 百脉根根际高效溶磷菌LC15的特性研究及菌株鉴定 |
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| 引用本文： | 蔡璐,马培杰,王小利,陈莹,陆瑞霞,王茜.百脉根根际高效溶磷菌LC15的特性研究及菌株鉴定[J].草原与草坪,2017,37(2). |
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| 作者姓名： | 蔡璐 马培杰 王小利 陈莹 陆瑞霞 王茜 |
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| 作者单位： | 1. 贵州省生物技术研究所,贵州 贵阳,550006;2. 贵州省草业研究所,贵州 贵阳,550006 |
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| 基金项目： | 贵州牧草根际溶磷菌库建立与溶磷机理研究 |
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| 摘 要： | 研究了分离自百脉根(Lotus corniculatus)根际的溶磷菌LC15,通过对其进行生理生化特性及16S rDNA序列分析表明,其菌株具有较强的溶解无机磷和有机磷的能力,且对无机磷的溶解能力更强,D/d比值均超过3.00;振荡培养7 d后其可溶性磷含量高达400.49 mg/L,分泌总有机酸量30.67 mmol/L,且菌悬液pH 4.39呈酸性;革兰氏染色呈阴性,在厌氧生长、V-P、柠檬酸盐利用、ONPG测定、精氨酸双水解酶等生理生化试验中均呈阳性;经BIOLOG测定,可利用碳源占95种供试碳源种类的54%;结合菌株16S rDNA序列同源性比对分析,初步鉴定为柠檬酸杆菌属(Citrobcter sp.)。
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| 关 键 词： | 溶磷菌 百脉根 溶磷能力 16SrDNA |
Characteristics and identification of phosphate-solubilizing bacteria LC15 isolated from rhizosphere of Lotus corniculatus |
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| CAI Lu,MA Pei-jie,WANG Xiao-li,CHEN Ying,LU Rui-xia,WANG Qian.Characteristics and identification of phosphate-solubilizing bacteria LC15 isolated from rhizosphere of Lotus corniculatus[J].Grassland and Turf,2017,37(2). |
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| Authors: | CAI Lu MA Pei-jie WANG Xiao-li CHEN Ying LU Rui-xia WANG Qian |
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| Abstract: | Phosphate-dissolving bacteria strain LC15 was isolated from the rhizosphere of Lotus corniculatus,and the physiological and biochemical characters and 16S rDNA sequence were analyzed.The results showed that LC15 had the strong ability of dissolving both inorganic and organic phosphorus,especially for inorganic phosphorus (D/d values were over 3.00);After 7 days incubation,the release of maximal available soluble phosphorus and organic acid reached to 400.49 mg/L and 30.67 mmol/L,respectively;The bacterial suspension was significantly acid (pH 4.39);Based on different measures,including Gram stain negative,anaerobic growth,V-P,citrate utilization,ONPG,arginine dihydrolase,BIOLOG et al.,as well as 16S rDNA sequence analysis,the strain LC15 was identified as Citrobcter sp..The strain has the great potential for developing the efficient microbial phosphate inoculant and microbial fertilizer. |
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| Keywords: | phosphate-dissolving bacteria Lotus corniculatus capacity of phosphate solubilization 16S rDNA |
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