| 血清2型鸭疫里默氏杆菌制苗用菌株的筛选及灭活油乳剂疫苗的研制 |
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| 引用本文： | 王小兰,胡青海,韩先干,丁铲,于圣青.血清2型鸭疫里默氏杆菌制苗用菌株的筛选及灭活油乳剂疫苗的研制[J].中国动物传染病学报,2012(1):54-58. |
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| 作者姓名： | 王小兰 胡青海 韩先干 丁铲 于圣青 |
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| 作者单位： | 中国农业科学院上海兽医研究所;国家家禽工程技术中心 |
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| 基金项目： | 上海市科技兴农重点攻关项目[沪农科攻字(2009)第5-3号];公益性行业(农业)科研专项经费项目(201003012) |
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| 摘 要： | 血清2型鸭疫里默氏杆菌(Riemerella anatipestifer,RA)分离株经雏鸭体内复壮、回收、鉴定后,选取其中2株细菌NJ3和Yb2进行毒力测定,结果表明其半数致死量分别为5.62×107CFU和1.07×105CFU。选取5株细菌制备灭活油乳剂疫苗后免疫鸭,并进行攻毒保护试验。结果表明不同菌株制备的疫苗均可使免疫鸭产生高水平的RA特异性ELISA抗体和攻毒保护力,但是攻毒保护性差异较大,NJ3免疫后的攻毒保护性最好。抗体检测及攻毒保护实验表明NJ-3是较理想的制苗用菌株。
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| 关 键 词： | 鸭疫里默氏杆菌 油乳剂灭活疫苗 ELISA |
SCREENING OF SEROTYPE 2 RIEMERELLA ANATIPESTIFER CANDIDATE STRAINS FOR THE DEVELOPMENT OF AN INACTIVATED OIL-EMULSION VACCINE |
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| WANG Xiao-lan,HU Qing-hai,HAN Xian-gan,DING Chan,YU Sheng-qing.SCREENING OF SEROTYPE 2 RIEMERELLA ANATIPESTIFER CANDIDATE STRAINS FOR THE DEVELOPMENT OF AN INACTIVATED OIL-EMULSION VACCINE[J].Chinese Journal of Animal Infectious Diseases,2012(1):54-58. |
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| Authors: | WANG Xiao-lan HU Qing-hai HAN Xian-gan DING Chan YU Sheng-qing |
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| Institution: | 1,2(1.Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;2.China National Engineering Technology Research Centre for Poultry,Shanghai 200331,China) |
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| Abstract: | Serotype 2 Riemerella anatipestifer strains were reisolated and characterized after inoculated into ducklings.Among obtained isolates,strains NJ3 and Yb2 were titrated in ducklings.The median lethal doses(LD50) were 5.62×107CFU for strain NJ3 and 1.07×105CFU for Yb2.Five reisolated strains(SC-2、Yb2、Th4、JY-1 and NJ-3) were selected to prepare inactivated oil-emulsion vaccines that were administered into Cherry Valley ducklings.All ducklings immunized with the above vaccines generated high ELISA titers.However,protection against challenge with either NJ3 or Yb2 showed significant difference among 5 candidate strains.The best protection in ducklings was obtained when NJ3 strain vaccine was used,indicating NJ-3 strain was an ideal candidate for the development of inactivated oil-emulsion vaccine. |
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| Keywords: | Riemerella anatipestifer oil-emulsion vaccine ELISA |
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