| 免疫鸡群H5N1和H9N2亚型禽流感病毒混合感染及H9N2亚型病毒进化分析 |
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| 引用本文： | 曾嘉梅,房栋,卞学兵,王俊桦,颜焰,周继勇.免疫鸡群H5N1和H9N2亚型禽流感病毒混合感染及H9N2亚型病毒进化分析[J].中国动物传染病学报,2012(2):65-70. |
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| 作者姓名： | 曾嘉梅 房栋 卞学兵 王俊桦 颜焰 周继勇 |
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| 作者单位： | 浙江大学农业部动物病毒学重点实验室;浙江大学传染病诊治国家重点实验室 |
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| 基金项目： | 国家科技支撑计划重点项目(2010BAD04B01);现代农业产业技术体系岗位专家项目(CARS-41-K11) |
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| 摘 要： | 本研究分析了免疫鸡群H5N1和H9N2亚型禽流感病毒混合感染中H9N2亚型分离毒株A/chicken/Yuyao/01/2010(H9N2)的基因组特征。氨基酸序列分析显示,HA蛋白裂解位点为PSRSSR/GL,具有低致病性禽流感病毒特征,HA受体结合位点出现了人流感病毒结合位点226L,D基因出现了S31N的突变。遗传分析表明,A/chicken/Yuyao/01/2010病毒的HA基因、NA基因和NS基因在进化上属于CK/BJ/94-Like谱系,D基因和PB2基因属于G1-Like谱系,NP基因、PA基因和PB1基因属于Ck/SH/F/98-Like谱系。结果表明,从H5N1和H9N2亚型混合感染鸡体内分离的H9N2亚型禽流感病毒是一株来源于CK/BJ/94-Like谱系、G1-Like谱系和Ck/SH/F/98-Like谱系的重组病毒。
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| 关 键 词： | 禽流感 混合感染 H9N2 分子进化分析 |
PHYLOGENETIC ANALYSIS OF H9N2 INFLUENZA VIRUSES CO-INFECTED WITH H5N1 VIRUSES IN VACCINATED CHICKENS |
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| ZENG Jia-mei,FANG Dong,BIAN Xue-bing,WANG Jun-hua,YAN Yan,ZHOU Ji-yong.PHYLOGENETIC ANALYSIS OF H9N2 INFLUENZA VIRUSES CO-INFECTED WITH H5N1 VIRUSES IN VACCINATED CHICKENS[J].Chinese Journal of Animal Infectious Diseases,2012(2):65-70. |
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| Authors: | ZENG Jia-mei FANG Dong BIAN Xue-bing WANG Jun-hua YAN Yan ZHOU Ji-yong |
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| Institution: | 1,2(1.Key Laboratory of Animal Virology of Ministry of Agriculture,Zhejiang University,Hangzhou310058,China;2.State Key Laboratory of Infectious Diseases for Diagnosis and Treatment,Zhejiang University,Hangzhou 310003,China) |
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| Abstract: | Avian influenza virus A/chicken/Yuyao/01/2010(H9N2) was isolated from chickens vaccinated,which were also infected with H5N1 virus.The whole genome of A/chicken/Yuyao/01/2010(H9N2) was sequenced and analyzed.The HA cleavage site was PSRSSR/GL,representing the character of low pathogenic influenza virus.The typical 226L of human-like influenza virus emerged in HA receptor binding site.The mutation S31N related to amantadine resistance was present in M gene.Phylogenetic analysis found that HA,NA and NS genes belonged to Ck/BJ/1/94-Like sub-clade,M and PB2 genes were from G1-Like lineage,and NP,PA and PB1 genes were from Ck/SH/F/98-Like lineage.All these data indicated that A/chicken/Yuyao/01/2010(H9N2) was a recombinant virus. |
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| Keywords: | Avian influenza mixed infection H5N1 H9N2 molecular evolution |
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