| 藏猪背最长肌中过氧化物酶体增殖物激活受体γ辅激活因子1α基因表达的发育性变化 |
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| 引用本文： | 蒋岸岸,李明洲,顾以韧,张凯,李学伟.藏猪背最长肌中过氧化物酶体增殖物激活受体γ辅激活因子1α基因表达的发育性变化[J].中国畜牧杂志,2010,46(5). |
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| 作者姓名： | 蒋岸岸 李明洲 顾以韧 张凯 李学伟 |
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| 作者单位： | 四川农业大学动物科技学院,四川,雅安,625014 |
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| 基金项目： | 国家公益性行业(农业)科研专项，国家现代农业产业技术体系专项 |
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| 摘 要： | 采用实时荧光定量PCR方法检测了藏猪背最长肌组织中过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARGC1A)基因mRNA表达量在2、4、6月龄和8月龄间的差异。结果表明:藏猪背最长肌中PPARGC1A基因mRNA的表达量在2月龄时最低,至4月龄时表达量极显著上升(P0.01),6月龄时表达量逐渐下降,但较4月龄差异不显著(P0.05),至8月龄时表达量又极显著上升到最高水平(P0.01)。本研究初步揭示了藏猪PPARGC1A基因的发育表达模式,为进一步研究PPARGC1A基因对猪肉质性状的影响提供基础数据。
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| 关 键 词： | 背最长肌 PPARGC1A 荧光实时定量PCR 猪 |
Developmental Expression Changes of PPARGC1A Gene in Longissimus Dorsi Muscle of the Tibetan Pigs |
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| JIANG An-an,LI Ming-zhou,GU Yi-ren,ZHANG Kai,LI Xue-wei.Developmental Expression Changes of PPARGC1A Gene in Longissimus Dorsi Muscle of the Tibetan Pigs[J].Chinese Journal of Animal Science,2010,46(5). |
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| Authors: | JIANG An-an LI Ming-zhou GU Yi-ren ZHANG Kai LI Xue-wei |
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| Abstract: | The expression changes of peroxisome proliferators activated receptor γ coactivator 1α (PPARGC1A) gene in longissimus dorsi muscle of Tibetan pigs at four postnatal growth stages (2,4,6 and 8 months) were measured by using real-time quantitative PCR approach.The results indicated that the mRNA abundance of PPARGC1A gene was lowest at 2-month,then strongly up-regulated to a higher level at 4-month (P < 0.01).After a slight down-regulation during 4-to 6-month (P > 0.05),the highest mRNA abundance was observed at 8-month(P < 0.01).These results highlight PPARGC1A as a possible candidate genes for pork quality traits and provide some data on which to base further study of the molecular basis of skeletal muscles growth. |
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| Keywords: | PPARGC1A |
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