| 鸡Mx基因2032位点单核苷酸多态性研究 |
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| 引用本文： | 吴晓伟,范敏其,倪黎纲,程旭梅,陈强,田智泉,李碧春.鸡Mx基因2032位点单核苷酸多态性研究[J].中国畜牧杂志,2009,45(5). |
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| 作者姓名： | 吴晓伟 范敏其 倪黎纲 程旭梅 陈强 田智泉 李碧春 |
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| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏,扬州,225009
2. 扬州大学动物科学与技术学院,江苏,扬州,225009;泰州畜牧兽医职业技术学院,江苏泰州,225300 |
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| 基金项目： | 国家自然科学基金，江苏省自然科学基金，高等学校博士学科点专项科研基金 |
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| 摘 要： | 研究采用错配PCR-RFLP的方法对15个品种鸡的Mx基因进行筛查,旨在探索不同鸡品种MxcDNA第2032位点抗病基因A与易感等位基因G的多态性,为生产转基因鸡的可行性提供理论基础。结果表明:15个鸡品种Mx基因的2032位点存在A、G2个等位基因,AA、AG、GG3种基因型,其中鹿苑鸡、狼山鸡、斗鸡未发现AA型个体,茶花鸡、红色原鸡未发现GG个体;携带Mx抗性基因A和易感基因G的比例分别是0.350、0.650;抗性等位基因A的检测比例为0.034~0.984。红色原鸡的抗性等位基因A基因频率(0.984)高于地方鸡种的抗性等位基因A的基因频率(0.321);地方鸡种中的观赏型、蛋肉兼用型、肉用型、蛋用型鸡种的抗性等位基因A的基因频率分别为0.221、0.297、0.425、0.462。χ2适合性检验表明,15个品种的鸡群均处于Hardy-Weinberg平衡状态。
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| 关 键 词： | Mx基因 抗性基因 单核苷酸多态性 鸡 |
Study on the Polymorphism of Mx Gene 2032 Position in Chicken |
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| WU Xiao-Wei,FAN Min-qi,NI Li-Gang,CHENG Xu-mei,CHEN Qiang,TIAN Zhi-quan,LI Bi-Chun.Study on the Polymorphism of Mx Gene 2032 Position in Chicken[J].Chinese Journal of Animal Science,2009,45(5). |
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| Authors: | WU Xiao-Wei FAN Min-qi NI Li-Gang CHENG Xu-mei CHEN Qiang TIAN Zhi-quan LI Bi-Chun |
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| Abstract: | A mismatch PCR-restriction fragment length polymorphism (PCR-RFLP) methods was used to study the 15 breeds of chicken Mx gene. The aim was to detect the difference of frequency of Mx resistance gene A and susceptibility gene G in different breeds. The results showed that 14 breeds of chicken Mx gene 2032 points existed two gene alleles, three kinds of genotype, which Luyuan chicken, Langshan chicken, Game chicken were found no AA genotype, Chahua chicken found no GG genotype; the average of resistance gene A and sensitive gene G was 0.358, 0.642. The frequency of resistance alleles A was from 0.034 to 0.846. The frequency of Mx resistance gene A in Red Jungle flow(0.984) was much higher than that of other native population (0.321). The 14 native breeds were divided into 4 types which include eggs typed, meat typed, both eggs and meat typed, watch typed, and their frequency of resistance Mx gene A were 0.221, 0.297, 0.425, 0.462, respectively. Meanwhile the result of Chi Square test indicated that all the populations were fit with Hardy-Weinberg equilibrium. |
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| Keywords: | Mx gene resistance gene single nucleotide polymorphism Chicken |
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