| 重庆板角山羊细管冷冻精液的研究 |
| |
| 引用本文： | 潘红梅,陈四清,王可甜,王金勇,刘勇,温清发,张凤鸣.重庆板角山羊细管冷冻精液的研究[J].中国畜牧杂志,2009,45(17). |
| |
| 作者姓名： | 潘红梅 陈四清 王可甜 王金勇 刘勇 温清发 张凤鸣 |
| |
| 作者单位： | 1. 重庆市畜牧科学院,重庆荣昌,402460;重庆市养猪工程中心,重庆荣昌,402460
2. 西南大学荣昌校区,重庆荣昌,402460 |
| |
| 基金项目： | 国家科技支撑计划项目，重庆市科技攻关计划项目 |
| |
| 摘 要： | 为了建立重庆板角山羊精液的细管冷冻保存方法,实验进行了不同冷冻稀释液(配方Ⅰ、Ⅱ、Ⅲ)、不同冷冻保存剂(甘油、EG)及不同离心速度(1000、1200、1400r/min)对重庆板角山羊细管精液冷冻保存效果的研究,结果表明:配方Ⅱ对重庆板角山羊精液的冻后活率显著优于配方Ⅰ和Ⅲ(P<0.05)。在配方Ⅱ中添加相同剂量(5%)的EG和甘油,精液冻后活率差异不显著(P>0.05)。以1200r/min的速度对山羊鲜精作离心处理后,冻后活率相对于对照组有所提高,但差异不显著(P>0.05)。
|
| 关 键 词： | 细管冷冻精液 EG 甘油 离心 重庆板角山羊 |
Study On Straw Frozen Semen of Chongqing Banjiao Goat |
| |
| PAN Hong-mei,CHEN Si-qing,WANG Ke-tian,WANG Jin-yong,LIU Yong,WENG Qing-fa,ZHANG Feng-ming.Study On Straw Frozen Semen of Chongqing Banjiao Goat[J].Chinese Journal of Animal Science,2009,45(17). |
| |
| Authors: | PAN Hong-mei CHEN Si-qing WANG Ke-tian WANG Jin-yong LIU Yong WENG Qing-fa ZHANG Feng-ming |
| |
| Abstract: | To establish the cryopreservation method of Chongqing Banjiao Goat semen by straws, different extender (extenderⅠ,Ⅱ,Ⅲ),different cryoprotectant(Glycerol and EG) and different centrifugal speed(1 000,1 200 r/min and 1 400 r/min) were studied. The results showed that:the extender Ⅱ was the best among the extenders and its sperm motility after freezing and thawing was significantly higher than that of other two extenders (P < 0.05); When added the same concentration (5%) of EG and glycerol into the extender II, sperm quality showed no significant difference (P > 0.05);Centrifuging semen at 1,200 to remove the seminal plasma before cryopreservation could slightly improve the frozen sperm motility. |
| |
| Keywords: | EG |
| 本文献已被 万方数据 等数据库收录！ |
|
