| 添加前缀对重组蛋白生物学特性影响的预测分析 |
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| 引用本文： | 李泽鸿.添加前缀对重组蛋白生物学特性影响的预测分析[J].中国兽药杂志,2010,44(12):32-35. |
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| 作者姓名： | 李泽鸿 |
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| 作者单位： | 1. 吉林农业大学生命科学学院,长春,130118
2. 吉林大学第一医院,长春,130021 |
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| 基金项目： | 长春市科技局资助课题项目 |
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| 摘 要： | 利用分子生物学软件DNASTAR对DAB389-αMSH和含有亲和标记、Xa factor识别序列、连接肽的DAB389（Gly4Ser）2-αMSH的表位、亲水性、等电点等生物学特性进行了预测分析。结果显示：DAB389（Gly4Ser）2-αMSH的表位为0.8,DAB389-αMSH为2.1;亲水性为2.7,高于DAB389-αMSH的1.96;两者的等电点均为5.9。His.Tag、Xa factor识别序列使重组蛋白的亲水性增强,表位降低;重组蛋白氨基端前70个氨基酸中不存在信号肽;DAB389（Gly4Ser）2-αMSH的表达量低于DAB389-αMSH2%左右,前缀对蛋白表达量有一定的影响。
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| 关 键 词： | 前缀 重组蛋白 亲水性 表位 信号肽 |
| 收稿时间： | 2010/8/19 0:00:00 |
| 修稿时间： | 9/5/2010 12:00:00 AM |
Predictive analysis in effect the bionomics of prefixing to recombination protein |
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| lizehong.Predictive analysis in effect the bionomics of prefixing to recombination protein[J].Chinese Journal of Veterinary Drug,2010,44(12):32-35. |
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| Authors: | lizehong |
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| Institution: | Jilin Agriculture University |
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| Abstract: | Abstract: The designed fusion protein DAB389(Gly4Ser)2-aMSH and DAB389-aMSH include affinity labeling, Xa factor, (Gly4Ser)2, was analyzed and compared in some molecular biology characteristics by using molecular biology software DNASTAR. The results showed that the epitope of DAB389(Gly4Ser)2-aMSH was 0.8 lower/less than 2.1 of DAB389-aMSH, it's hydrophilicity was 2.7 higher than 1.96 of DAB389-aMSH, The isoelectric point of them was 5.9. His×Tag, Xa factor recognition sequence made the hydrophilicity strengthen, epitope and antigenicity depress; the pro 70 amino acid in N-terminal of recombination protein had no signal peptide, the expression quantity of DAB389(Gly4Ser)2-aMSH was less 2% than that of DAB389-aMSH, these prefixing had determinate influence to expression protein. |
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| Keywords: | prefix Recombination protein Hydrophilicity Epitope Signal peptide |
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