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| 白鳍鲨雷极氏普罗菲登斯菌的分离鉴定与药敏试验 | |
| 引用本文： | 李伟杰.白鳍鲨雷极氏普罗菲登斯菌的分离鉴定与药敏试验[J].中国兽药杂志,2010,44(6):48-50. |
| 作者姓名： | 李伟杰 |
| 作者单位： | 1. 中国兽医药品监察所,北京,100081 2. 北京动物园,北京,100044 3. 北京海洋馆,北京,100044 |
| 摘 要： | 从北京海洋馆死亡的白鳍鲨腹腔脓液和心血中分离到一株病原菌,经生理生化、培养特性、16S rRNA序列鉴定为雷极氏普罗菲登斯菌。以16S rRNA序列为基础构建了包括普罗菲登斯菌属模式株在内的系统发育树,分离菌株与雷极氏普罗菲登斯菌模式株DSM4542的同源性最近。药敏试验结果表明,该菌对氨曲南、头孢曲松、磷霉素、哌拉西林、头孢呋辛钠、诺氟沙星、头孢哌酮敏感。毒力试验结果显示该菌可致小鼠死亡。 |
| 关 键 词： | 雷极氏普罗菲登斯菌 16SrRNA序列 药敏试验 毒力试验 |
| 收稿时间： | 2010/2/10 0:00:00 |
| 修稿时间： | 2010/4/16 0:00:00 |
Identification of Providencia rettgeri isolated from Whitetip shark |
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| LI Weijie.Identification of Providencia rettgeri isolated from Whitetip shark[J].Chinese Journal of Veterinary Drug,2010,44(6):48-50. | |
| Authors: | LI Weijie |
| Institution: | Chian Institute of Veterinary Drug Control |
| Abstract: | A pathogenic bacteria isolated from the dead whitetip shark in Beijing aquarium were identified as Providencia rettgeri by biochemical and physillogical characteristics, culture characteristics, 16S rRNA sequence analysis. A phylogenetic tree was construced by comparing with the validly published 16S rRNA sequence of the related type strains from GenBank. The result shows that the strain I belongs to Providencia rettgeri. Drug sensitivity test shows that the strain is sensitive to Aztreonam, Ceftriaxone, Fosfomycin , Piperacillin , Cefuroxime Sodium , Norfloxacin , Cefoperazone. The result of virulence test shows that the strain I can cause mice dead. |
| Keywords: | 16S |
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