| 水貂和狐犬瘟热病毒F基因的克隆与序列分析 |
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| 引用本文： | 袁小远,单虎,王志亮.水貂和狐犬瘟热病毒F基因的克隆与序列分析[J].经济动物学报,2005,9(3):129-131. |
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| 作者姓名： | 袁小远 单虎 王志亮 |
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| 作者单位： | 山东省农业科学院家禽研究所,济南,250100;农业部动物检疫所国家外来动物疫病诊断中心,青岛,266032;山东莱阳农学院动物科技学院,青岛,266109;农业部动物检疫所国家外来动物疫病诊断中心,青岛,266032 |
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| 摘 要： | 为探寻免疫失败的原因,对山东某养殖场免疫后的发病水貂和狐犬瘟热组织病料分别提取RNA进行RT-PCR扩增。将PCR得到的部分F基因克隆到pMD18-T载体上测序,并与疫苗株ND进行序列分析。结果表明,水貂和狐的CDV与OND的核苷酸同源性为98.6%9、8.6%,氨基酸同源性为98.0%9、8.0%。水貂和狐两者核苷酸同源性为98.0%,氨基酸同源性为95.9%。
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| 关 键 词： | 水貂 狐 犬瘟热病毒 序列分析 |
| 文章编号： | 1007-7448(2005)03-0129-03 |
| 收稿时间： | 2005-06-02 |
| 修稿时间： | 2005年6月2日 |
Cloning and Sequencing Analysis of F Gene of Canine Distemper Virus from Mink and Fox |
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| YUAN Xiao-yuan,SHAN Hu,WANG Zhi-liang.Cloning and Sequencing Analysis of F Gene of Canine Distemper Virus from Mink and Fox[J].Journal of Economic Animal,2005,9(3):129-131. |
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| Authors: | YUAN Xiao-yuan SHAN Hu WANG Zhi-liang |
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| Abstract: | The F gene of canine distemper virus was amplified by RT-PCR from tissues of mink and fox which had been immunized and clinically suspected of canine distemper in Shandong. The segments of F gene obtained by R T-PCR were cloned into pMD18-T vector and sequenced. The sequences were analyzed homologically in comparison with OND strain of CDV and the results indicated that CDV strains from ferret , fox and OND strain shared 98,6%, 98.6% homology respectively and the deduced amino acid sequence had 98.0%, 98.0% identities correspondingly. The sequence analysis of the gene of CDV from fox and mink tissue showed that they shared 98.0% homology in nucleotide sequence and 95.9%. in the deduced amino acid sequence. |
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| Keywords: | mink fox canine distemper vires sequence analysis |
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