| 1株大肠杆菌O157鸭源噬菌体的分离、纯化及其特性鉴定 |
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| 引用本文： | 王雪松,李晓,杨光远,刘鹏刚,汤芳,张炜,陆承平.1株大肠杆菌O157鸭源噬菌体的分离、纯化及其特性鉴定[J].畜牧与兽医,2011,43(7). |
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| 作者姓名： | 王雪松 李晓 杨光远 刘鹏刚 汤芳 张炜 陆承平 |
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| 作者单位： | 南京农业大学动物医学院,江苏南京,210095 |
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| 基金项目： | 国家大学生创新性试验计划(091030713) |
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| 摘 要： | 将安徽某鸭场的400份鸭粪样品离心,微孔滤器过滤后提取上清液,将上清液与均匀涂布于LB固体培养基上的大肠杆菌O157ATCC43889滴定,6 h后初步鉴定噬菌体。将疑似存在噬菌体的上清液采用双层琼脂平板法进一步鉴定,结果分离鉴定了1株噬菌体,命名为EC43889-30。3次纯化后得到直径约为2 mm的噬菌斑,富集得到纯培养物。电镜观察,噬菌体粒子无尾,为直径50 nm左右的正六边形,形态与复层病毒科(Tectiviridae)的成员相似。将纯培养物稀释至107 pfu/mL,每隔10 min(至50 min)分别测其在50℃,60℃和70℃时噬菌体存活数,热稳定曲线显示,50℃时存活率均在70%左右,60℃时存活率明显下降,70℃时完全失活。宿主谱检测结果显示,其宿主范围较窄,对测试的42株鸭源大肠杆菌无裂解作用。
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| 关 键 词： | 大肠杆菌O157 噬菌体 分离 特性 |
Isolation, purification and characterization of an E.coli O157 bacteriophage from duck faeces |
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| WANG Xue-song,LI Xiao,YANG Guang-yuan,LIU Peng-gang,TANG Fang,ZHANG Wei,LU Cheng-ping.Isolation, purification and characterization of an E.coli O157 bacteriophage from duck faeces[J].Animal Husbandry & Veterinary Medicine,2011,43(7). |
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| Authors: | WANG Xue-song LI Xiao YANG Guang-yuan LIU Peng-gang TANG Fang ZHANG Wei LU Cheng-ping |
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| Institution: | WANG Xue-song,LI Xiao,YANG Guang-yuan,LIU Peng-gang,TANG Fang,ZHANG Wei,LU Cheng-ping(College of Veterinary Medicine,Nanjing Agriculture University,Nanjing 210095,China) |
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| Abstract: | Four hundreds of faecal samples from ducks in Anhui Province were centrifugated and filtered by 0.22um filters.The supernatants were titrated on LB solid medium pooled with E.coli O157 ATCC43889 and the plaques were picked up after 6 hours.By using the double-deck agar,a strain of bacteriophage named EC43889-30 was isolated.After purification for three times,the plaques in 2mm diameter were enriched.The electron microscopic observation revealed that the bacteriophage had an autho-sexangle head with approxim... |
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| Keywords: | E coli O157 bacteriophage isolation characterization |
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