| 河南省羊肠道病毒的分离与序列测定 |
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| 引用本文： | 王汝都,林倩,李欣,常晓冉,胡俊英,郑博伟,张泽财,朱金凤,王新平.河南省羊肠道病毒的分离与序列测定[J].畜牧与兽医,2020(5):112-116. |
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| 作者姓名： | 王汝都 林倩 李欣 常晓冉 胡俊英 郑博伟 张泽财 朱金凤 王新平 |
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| 作者单位： | 吉林大学动物医学学院;河南农业职业学院;沈阳工学院生命工程学院 |
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| 基金项目： | “十三五”国家重点研发计划资金项目(2016YFD0500904,2017YFD0500104);河南省高等教育教学改革研究与实践项目(2017SJGLX152)。 |
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| 摘 要： | 应用Vero细胞从河南省某腹泻羊场的粪便样品中分离出2株病毒。电镜观察显示,分离的病毒粒子直径大小为25~30 nm。单层免疫过氧化物酶对分离病毒进行了免疫学鉴定,结果显示分离的2株病毒均与羊肠道病毒CEV-JL14的高免血清发生强阳性反应。对RT-PCR扩增出的病毒基因片段进行了序列测定与比对分析,发现分离的2株病毒与CEV-JL14毒株处于同一分支,均为G种肠道病毒。本研究首次从河南省分离获得了羊肠道病毒,为进一步研究该病打下基础。
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| 关 键 词： | 羊肠道病毒 分离鉴定 G种肠道病毒 核苷酸序列比对 变异进化 |
Isolation and sequencing of the enterovirus from goat herds in Henan Province |
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| WANG Rudu,LIN Qian,LI Xin,CHANG Xiaoran,HU Junying,ZHENG Bowei,ZHANG Zecai,ZHU Jinfeng,WANG Xinping.Isolation and sequencing of the enterovirus from goat herds in Henan Province[J].Animal Husbandry & Veterinary Medicine,2020(5):112-116. |
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| Authors: | WANG Rudu LIN Qian LI Xin CHANG Xiaoran HU Junying ZHENG Bowei ZHANG Zecai ZHU Jinfeng WANG Xinping |
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| Institution: | (College of Veterinary Medicine,Jilin University,Changchun 130062,China;Henan Vocational College of Agriculture,Zhengzhou 451450,China;College of Life Engineering,Shenyang Institute of Technology,Fushun 113122,China) |
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| Abstract: | Two enterovirus strains were isolated,using Vero cells,from the fecal samples from the goat herds infected with diarrhea in Henan Province.Observation by electron microscopy showed that the isolated viruses had a particle size of approximately 25-30 nm in diameter.The results from immunoperoxidase monolayer assay(IPMA)indicated that the two viruses had a strong immune cross-reaction with the antibody against the CEV-JL14 enterovirus.Analysis of the nucleotide sequences amplified from the isolated viruses showed the viruses contained a sequence in high homology with the Caprine enterovirus CEV-JL14.Phylogenetic analysis clustered the two viruses with CEV-JL14.To our knowledge,this has been the first report of the isolation of Caprine enterovirus from the goat herd in Henan Province,which laid a basis for future study of Caprine enterovirus infection. |
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| Keywords: | Caprine enteroviruses isolation and identification enterovirus G phylogenetic analysis variation evolution |
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