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| 云南2株H4N6亚型禽流感病毒株的分离与鉴定 | |
| 引用本文： | 李昀海,赵焕云,张文东,吕粤,岳亮,宋建领,范泉水,张应国,张富强.云南2株H4N6亚型禽流感病毒株的分离与鉴定[J].畜牧与兽医,2011,43(6). |
| 作者姓名： | 李昀海 赵焕云 张文东 吕粤 岳亮 宋建领 范泉水 张应国 张富强 |
| 作者单位： | 1. 云南农业大学,云南昆明,650220 2. 云南省动物疫病预防控制中心,云南昆明,650051 3. 云南省热带亚热带动物病毒病重点实验室,云南昆明,650224 4. 成都军区疾病预防控制中心,云南昆明,650032 |
| 基金项目： | 国家农业公益性行业科研专项 |
| 摘 要： | 采集家禽喉气管作为检测样品,用甲型流感病毒M基因检测引物进行RT-PCR检测,确定样品为甲型流感病毒感染。用已建立的PCR法检测H5、H9亚型及新城疫结果为阴性。在9日龄鸡胚中进行病毒分离培养,收集48 h后死亡鸡胚尿囊液进行血凝和血凝抑制试验,有血凝效价但不能被H5、H7、H9和新城疫阳性血清抑制。为进一步确定病毒的亚型,用甲型流感病毒血凝素基因通用引物进行RT-PCR扩增、克隆及测序,将测得的HA基因序列与血凝素亚型标准序列进行比对,同源性为34.9%～87.3%,与H4亚型的同源性为87.3%;设计神经氨酸酶基因测序引物,将所测序列与神经氨酸酶亚型标准序列进行比对,同源性为47.7%～90.8%,与N6亚型的同源性高达90.8%。通过进行HA和NA基因序列比对,可以确定试验所分离到的2株甲型流感毒株为H4N6亚型禽流感病毒。 |
| 关 键 词： | 禽流感病毒 H4N6亚型 鉴定 云南 |
Isolation and identification of 2 strains of H4N6 avian influenza virus in Yunnan Province |
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| LI Yun-hai,ZHAO Huan-yun,ZHANG Wen-dong,LV Yue,YUE Liang,SONG Jian-ling,FAN Quan-shui,ZHANG Ying-guo,ZHANG Fu-qiang.Isolation and identification of 2 strains of H4N6 avian influenza virus in Yunnan Province[J].Animal Husbandry & Veterinary Medicine,2011,43(6). | |
| Authors: | LI Yun-hai ZHAO Huan-yun ZHANG Wen-dong LV Yue YUE Liang SONG Jian-ling FAN Quan-shui ZHANG Ying-guo ZHANG Fu-qiang |
| Institution: | LI Yun-hai1,ZHAO Huan-yun2,ZHANG Wen-dong2,LV Yue2,YUE Liang2,SONG Jian-ling3,FAN Quan-shui4,ZHANG Ying-guo2,ZHANG Fu-qiang4(1.Yunnan Agriculture University,Kunming 650220,China,2.Centre for Animal Disease Prevention and Control,Kunming 650051,3.Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory,Kunming 650224,4.Centre for Disease Prevention and Control,Cheng Du Military Region,Kunming 650032,China) |
| Abstract: | Two laryngotracheal samples were collected and demonstrated for the infection of avian influenza virus(AIV) by the RT-PCR amplification of M gene.The samples were further verified negative for the H9,H5 subtype AIV by the established PCR methods.The 11-day-old chicken embryonated eggs were used to cultivate the virus,and then the allantoic fluid was collected for the hemagglutination and hemagglutination inhibition assays.The positive blood sera of H5,H7 and H9 subtype AIV and Newcastle disease virus could ... |
| Keywords: | Avian influenza virus H4N6 identification Yunnan province |
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