| 猪源奇异变形杆菌的分离鉴定、耐药性分析及毒力基因检测 |
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| 引用本文： | 黎娜铭,王彬,舒正强,曾智勇,黄涛,汤德元,吴学祥.猪源奇异变形杆菌的分离鉴定、耐药性分析及毒力基因检测[J].畜牧与兽医,2021(3):89-94. |
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| 作者姓名： | 黎娜铭 王彬 舒正强 曾智勇 黄涛 汤德元 吴学祥 |
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| 作者单位： | 贵州大学动物科学学院;贵州省独山县麻尾镇人民政府;贵州省黔西南州农业农林局 |
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| 基金项目： | 黔科合支撑[2019]2276号;黔科合支撑[2020]1Y033号项目资助。 |
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| 摘 要： | 为确定屠宰场发病猪死亡原因,本研究对从死亡猪内脏中分离到的细菌,进行革兰染色镜检、生化鉴定和16S rRNA基因测序鉴定,并进行药敏试验、毒力基因检测及小鼠攻毒试验。结果显示,革兰染色镜检为细小或中等短杆状或丝状革兰阴性杆菌;生化试验特性分析及16S rRNA基因测序鉴定为奇异变形杆菌,命名为GZ2-2株。药敏试验结果显示,分离菌对哌拉西林、头孢呋辛、头孢他啶等11种药物敏感,对卡那霉素和米诺环素2种药物为中敏,对氨苄西林、头孢唑林、四环素等6种药物耐药;毒力基因检测结果显示,该分离菌携带rsbA、atfA、pmfA、mrpA、zapA、ureC、atfC和ucaA 8种毒力基因;小鼠攻毒试验表明,该分离菌有较强致病力。本试验成功分离到一株具有多重耐药性并携带8种毒力基因的奇异变形杆菌,为该菌感染的防治及其分子流行病学研究奠定了基础。
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| 关 键 词： | 猪 奇异变形杆菌 分离鉴定 耐药性 毒力基因 |
Isolation and identification of swine-sourced Proteus mirabilis,and detection of its drug resistance and virulence genes |
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| LI Naming,WANG Bin,SHU Zhengqing,ZENG Zhiyong,HUANG Tao,TANG Deyuan,WU Xuexiang.Isolation and identification of swine-sourced Proteus mirabilis,and detection of its drug resistance and virulence genes[J].Animal Husbandry & Veterinary Medicine,2021(3):89-94. |
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| Authors: | LI Naming WANG Bin SHU Zhengqing ZENG Zhiyong HUANG Tao TANG Deyuan WU Xuexiang |
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| Institution: | (College of Animal Science,Guizhou University,Guiyang 550025,China;People’s Government of Mawei Town,Dushan County,Guizhou Province,Dushan 558206,China;Agricultural and Rural Bureau of Qianxinan Prefecture,Guizhou Province,Xingyi 562400,China) |
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| Abstract: | In order to determine the cause of death of pigs in the slaughterhouse,a bacterium was isolated from the offal of dead pigs,was subjected to test under Gram stain microscopy,biochemical identification,16 S rRNA gene sequencing,drug sensitivity test,virulence gene detection and pathogenicity test in mice.The results were that Gram staining microscopic examination showed the presence of fine or medium-short rod-shaped or filamentous Gram-negative bacilli,and that characteristics analysis by biochemical test and 16 S rRNA gene sequencing identified the isolate as Proteus mirabilis,which we named the GZ2-2 strain.The susceptibility test results showed that the isolates were resistant to six drugs,such as ampicillin,cefazolin,and tetracycline,and so on;moderately sensitive to two drugs:kanamycin and minocycline;and sensitive to 11 drugs,such as piperacillin,cefuroxime,ceftazidime,and so on.The virulence gene test results showed that the isolate carried eight virulence genes:rsbA,atfA,pmfA,mrpA,zapA,ureC,atfC and ucaA.The pathogenicity test in mice showed that the isolate had strong pathogenicity.Therefore,a strain of Proteus mirabilis(GZ2-2)with multi-drug resistance and carrying eight virulence genes was successfully isolated in this study,which laid a foundation for prevention and control of Proteus mirabilis and for molecular epidemiology research on the strain. |
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| Keywords: | pig Proteus mirabilis isolation and identification drug susceptibility test virulence genes |
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