| 刘晓慧, 周连柱, 孔繁芳, 王忠跃, 黄晓庆*, 张昊*.中国4省份葡萄霜霉病菌群体对烯酰吗啉的抗性及适合度[J].植物保护,2023,49(3):172-178. |
| 中国4省份葡萄霜霉病菌群体对烯酰吗啉的抗性及适合度 |
| Resistance of Plasmopara viticola populations to dimethomorph in four provinces, China and their fitness |
| 投稿时间：2022-03-28 修订日期：2022-06-25 |
| DOI：DOI: 10.16688/j. zwbh. 2022161 |
| 中文关键词: 葡萄霜霉病菌 烯酰吗啉 抗药性 适合度 |
| 英文关键词:Plasmopara viticola dimethomorph fungicide resistance fitness |
| 基金项目:国家现代农业产业技术体系建设专项（CARS-29）；国家自然科学基金（32001843） |
|
| 摘要点击次数: 180 |
| 全文下载次数: 260 |
| 中文摘要: |
| 为明确我国山西、山东、河北和云南省葡萄霜霉病菌对烯酰吗啉的抗性以及田间病菌群体的适合度, 本研究采用Taqman MGB技术检测了2020年－2021年采自上述4省份292株葡萄霜霉病菌对烯酰吗啉的抗性频率; 采用叶盘法测定了田间抗性菌株与敏感菌株的适合度。结果表明, 上述4个地区的平均烯酰吗啉抗性频率及抗性等位基因频率分别为64.0%和74.7%, 其中山东省蓬莱区最高(91.2%和96.3%), 河北省廊坊市(73.8%和85.0%)和云南省宾川县(75.0%和82.6%)次之, 山西省清徐县(16.7%和34.7%)最低。适合度分析发现, 田间抗性菌株的产孢能力明显高于敏感菌株, 但二者的复合适合度指数无显著差异。上述结果表明, 4省份霜霉病菌群体均对烯酰吗啉产生了不同程度的抗性, 田间抗性菌株与敏感菌株相比无明显适合度变化。 |
| 英文摘要: |
| In order to clarify the resistance of Plasmopara viticola field populations to dimethomorph in Shanxi, Shandong, Hebei and Yunnan provinces of China and their fitness, the dimethomorph resistance of 292 P. viticola isolates collected from 2020 to 2021 was determined by Taqman MGB real time PCR, and the fitness of resistant and sensitive isolates was determined on detached leaves. The results showed that the average resistance frequency and resistance allele frequency of P. viticola from the four provinces were 64.0% and 74.7%, respectively. Among them, the average resistance frequency and resistance allele frequency in the populations of Penglai, Shandong province were the highest (91.2% and 96.3%), followed by those in Langfang of Hebei province (73.8% and 85.0%) and Binchuan of Yunnan province (75.0% and 82.6%), and the lowest was detected in Qingxu of Shanxi province (16.7% and 34.7%). The sporulation capacity of the resistant isolates was significantly higher than that of the sensitive ones, but there was no significant difference in the composite fitness index between them. The results indicated that the resistance of P. viticola populations to dimethomorph in the four provinces has developed to various degrees, but no significant difference existed in the fitness between sensitive and resistant isolates. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
