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1.
中草药复方制剂对公猪精液品质和生殖激素的影响 总被引：2，自引：0，他引：2
为了研究中草药复方制剂对公猪精液品质和生殖激素的影响,对27头精液品质相近的成年大约克种公猪饲喂不同剂量的中草药复方制剂,测定精液指标和血清生殖激素水平。结果表明:与对照组相比,2%低剂量组(试验Ⅰ组)的采精量、采精持续时间、精子密度,血清中促卵泡刺激素(FSH)、促黄体生成素(LH)和睾酮(T)含量差异显著(P<0.05);与对照组相比,3%高剂量组(试验Ⅱ组)的采精持续时间、精子活力和血液中LH、T含量显著提高,采精量和血液中FSH含量差异极显著(P<0.01);高、低剂量组的精子畸形率与对照组相比均有所降低。说明日粮中添加一定剂量中草药复方制剂可显著提高种公猪的精液品质,促进生殖激素的分泌。 相似文献
2.
本试验通过在饲粮中添加维生素与矿物质、调整饲粮能量蛋白质水平,旨在研究其对浙东白鹅母鹅繁殖性能、血液生殖激素浓度和生殖轴相关基因mRNA相对表达量的影响.选择138只月龄相近的浙东白鹅种母鹅,按体重相近原则分为3组,分别饲喂不同的饲粮,试验期150 d,测定繁殖性能(平均产蛋数、平均蛋重、受精率和孵化率)、血液生殖激素[卵泡刺激素(FSH)、促黄体生成素(LH)、孕酮(P4)、雌二醇(E2)、催乳素(PRL)]浓度和生殖轴相关基因[促性腺激素释放激素(GnRH)、卵泡刺激素-β(FSHβ)、雌激素受体1(ESR1)、雌激素受体2(ESR2)、卵泡刺激素受体(FSHR)、催乳素(PRL)、催乳素受体(PRLR)] mRNA相对表达量的变化.结果表明:1)添加维生素与矿物质可显著提高浙东白鹅母鹅第1产蛋周期平均蛋重和受精率(P＜0.05);提高第2产蛋周期内血液FSH和P4的浓度,降低LH浓度,改变E2、P4和PRL浓度波动(P＜0.05);下调下丘脑PRLR、垂体PRL和卵巢PRLR基因的mRNA相对表达量(P＜0.05),上调卵巢ESR2基因的mRNA相对表达量(P＜0.05).2)调整饲粮能量蛋白质水平可显著提高浙东白鹅母鹅第2产蛋周期平均蛋重(P＜0.05);提高浙东白鹅第2产蛋周期内血液LH浓度,降低FSH浓度,改变E2和P4浓度波动(P＜0.05);上调下丘脑GnRH、垂体PRL和PRLR基因的mRNA相对表达量(P＜0.05),下调卵巢FSHR基因的mRNA相对表达量(P＜0.05).由此得出,添加维生素与矿物质、调整饲粮能量蛋白质水平可通过影响产蛋周期内部分血液生殖激素浓度和波动,局部调节生殖轴相关基因的mRNA相对表达量,改善浙东白鹅母鹅的繁殖性能. 相似文献
3.
旨在研究春季对小尾寒羊、蒙古羊进行同期发情与人工授精处理时,不同外源激素使用对两品种羊繁殖指标和血液生殖激素浓度的影响。挑选春季小尾寒羊、蒙古羊母羊各120只,按不同外源激素处理方法随机均分为4组,其中对照组处理方法为阴道氟孕酮海绵栓(FGA)+撤栓后即注射孕马血清促性腺激素(PMSG),试验Ⅰ组为FGA+撤栓前24 h注射PMSG,试验Ⅱ组为FGA+撤栓前24 h注射PMSG+注射促黄体素释放激素A3(LHRH-A3),试验Ⅲ组为FGA+撤栓前24 h注射PMSG+注射LHRH-A3+稀释精液中添加催产素(OXT)。结果：试验I组与对照组相比,蒙古羊放栓第14天孕激素(P4)浓度显著升高(P<0.05),卵泡刺激素(FSH)浓度和发情反应率均极显著升高(P<0.01);试验Ⅱ组与试验Ⅰ组相比,小尾寒羊产羔率极显著提高(P<0.01),蒙古羊放栓第14天FSH、促性腺激素释放激素(GnRH)浓度和同期受胎率均极显著升高(P<0.01),同期妊娠率显著提高(P<0.05)... 相似文献
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5.
试验旨在研究热应激对金定鸭生产性能、血液生殖激素、卵巢生殖相关基因表达量及免疫指标的影响。试验选用产蛋期400日龄的金定鸭,设置对照组（常温组）、试验组（热应激）。对照组环境温度（25±2）℃,试验组环境温度（35±2）℃;测定热应激下金定鸭生产指标、血清生殖激素指标和免疫指标、卵巢生殖基因表达量。结果显示：与对照组相比,试验组产蛋率、总蛋重和平均蛋重极显著降低（P<0.01）;血清促卵泡生成素（FSH）含量显著降低（P<0.05）;卵巢促性腺素释放激素（GnRH）基因表达量显著降低（P<0.05）,促黄体生成素（LH）基因表达量极显著降低（P<0.05）;血清IgG含量极显著降低（P<0.01）,肿瘤坏死因子-α(TNF-α)含量显著升高（P<0.05）。由此可见,热应激对金定鸭生产性能、生殖激素水平及免疫指标产生不利影响,其中产蛋率、总蛋重、平均蛋重、血清FSH及卵巢LH、GnRH基因表达量均显著降低,蛋鸭炎性因子水平显著增加,机体免疫力显著下降。 相似文献
6.
抑制素pCIS基因免疫对黄牛卵泡发育和生殖激素的影响 总被引：2，自引：1，他引：1
分别用O(C)、0.75(T1)、1.50(T2)、2.25(T3)和3.0(T4)nag·头'-1的抑制素pCIS基因疫苗免疫58头黄牛,以探讨抑制素pCIS基因免疫对卵泡发育和生殖激素的影响.结果表明,抑制素pCIS基因免疫组的大卵泡(d≥10 mm)数显著高于对照组(P<0.01),中卵泡(7 mm≤d<10 mm)数和小卵泡(4 mm≤d<7 mm)数与对照组均没有显著差异(P>0.05),T1组左右两侧成熟卵泡大小与对照组差异不显著(P>0.05),其它3组两侧成熟卵泡均显著大于对照组(P<0.05).抑制素pCIS基因免疫黄牛的促卵泡素(FSH)平均含量高于对照组,且在加强免疫阶段差异显著(P<0.05);加强免疫后雌二醇(E2)和孕酮(P4)含量均显著增加,且均与对照组差异显著(P<0.05).这些结果表明,抑制素pCIS基因免疫可促进FSH分泌,进而影响黄牛卵泡发育和成熟. 相似文献
7.
本研究旨在比较1、3月龄绵羔羊激素处理后卵巢、子宫及血液中促卵泡激素(FSH)变化,研究不同月龄对羔羊卵泡发育的影响。通过对1、3月龄羔羊进行FSH和孕马血清促性腺激素(PMSG)处理,比较羔羊在激素处理前后血液中FSH水平和卵巢、子宫大小变化,卵巢上2～8 mm卵泡的数量和卵母细胞体外成熟、受精后胚胎的发育情况。结果表明:1月龄羔羊实验组体内整体FSH水平高于3月龄羔羊(P<0.05)。1月龄羔羊注射外源激素后两侧卵巢可获卵母细胞数(42.3±2.5、36.8±1.1)枚及体外受精囊胚发育率(16.33%±0.96%)显著高于3月龄羔羊卵母细胞数(10.0±0.7、8.5±0.6)枚及囊胚率(9.29%±1.55%)(P<0.05)。羔羊进行超数排卵处理时卵巢上卵泡发育与血液中FSH水平密切相关,且较高的FSH水平预示着较多的卵泡发育。 相似文献
8.
《黑龙江畜牧兽医》2017,(21)
为了探讨硒代蛋氨酸对蛋用种公鸡繁殖性能以及血液生殖激素的影响,试验选取180只140日龄京粉蛋用种公鸡,在其日粮中添加不同水平硒代蛋氨酸,最终使硒元素浓度达到0,0.25,0.5,1,2 mg/kg,于试验期末检测精液品质,统计种蛋受精率和孵化率,同时采集血液进行生殖激素含量的测定。结果表明:与对照组相比,1 mg/kg处理组精子活率显著提高5.66%(P0.05);0.5 mg/kg处理组精液量、精子密度、精子活力分别显著提高42.11%、38.53%、21.25%(P0.05);0.5 mg/kg处理组种蛋受精率提高了2.65%(P0.05);1 mg/kg处理组种蛋孵化率显著提高了2.22%(P0.05)。与对照组相比,1 mg/kg处理组血液中睾酮(T)显著提高了50.88%(P0.05);0.5 mg/kg处理组促黄体素(LH)显著提高了35.47%(P0.05);1 mg/kg处理组促卵泡素(FSH)提高了25.66%(P0.05)。说明硒代蛋氨酸对蛋用种公鸡精液品质、血液生殖激素及种蛋受精率、孵化率有较大影响,硒水平达到1 mg/kg时效果最佳。 相似文献
9.
《中国畜牧杂志》2017,(4)
实验旨在研究美洲大蠊提取物是否对大鼠卵巢卵泡发育产生影响。将成年SD大鼠随机分为3组,对照组(9 m L/kg,生理盐水)5只,低剂量组(9 m L/kg,0.03 g/m L美洲大蠊提取物)6只、高剂量组(9 m L/kg,0.09 g/m L美洲大蠊提取物)6只。每隔1 d灌胃一次,30 d后采集卵巢。结果表明:与对照组相比,低剂量组大鼠的卵巢重和卵巢体重比以及高剂量组大鼠的体重和卵巢重均显著增加(P0.05);高、低剂量组大鼠卵巢原始卵泡的数目均显著减少(P0.05),成熟卵泡显著增多(P0.05);生长发育相关基因BMP4、GDF9的表达显著上调(P0.05);低剂量组IGF-Ⅰ基因的表达有升高的趋势(P=0.058),但高剂量组则显著上调(P0.05);3个组间凋亡相关基因Bcl-2、BAX、Fas、Fas L和Caspase-3的表达均无显著性差异;但高剂量组使增殖相关基因的表达有升高的趋势(P=0.06),使Ki67基因的表达显著升高PCNA(P0.05)。本实验条件下,美洲大蠊提取物能够加快大鼠卵泡的发育,促进卵巢卵泡的成熟。 相似文献
10.
《黑龙江畜牧兽医》2015,(23)
为了筛选提高产蛋后期蛋鸡生产性能的适宜中草药添加剂剂量,试验采用单因子设计方法,选取健康、体重基本一致的430日龄海兰褐蛋鸡900只,随机分为3组,对照组饲喂基础日粮,试验1,2组分别添加2,1 g/(只·d)中草药添加剂,测定鸡生产性能及外周血清激素含量。结果表明:试验1组蛋鸡产蛋率比对照组提高8.1%,蛋重增加3.77 g,料蛋比降低10.37%,差异显著或极显著(P0.05或P0.01);添加中草药添加剂45天,试验1,2组蛋鸡血清中促卵泡生成素(FSH)、促黄体生成素(LH)、雌二醇(E2)、孕激素(P)和胰岛素生长因子(IGF-Ⅰ)含量分别比对照组高0.88,0.61 m IU/m L、0.13,0.06 m IU/m L、320.34,204.00 pg/m L、4.95,2.91 nmol/L及53.33,16.33 ng/m L。说明中药添加剂适宜添加剂量为2 g/(只·d)。 相似文献
11.
Yousuke NANIWA Keisuke NAKATSUKASA Shohei SETSUDA Shinya OISHI Nobutaka FUJII Fuko MATSUDA Yoshihisa UENOYAMA Hiroko TSUKAMURA Kei-ichiro MAEDA Satoshi OHKURA 《The Journal of reproduction and development》2013,59(6):588-594
Kisspeptin is a key molecule that stimulates gonadotropin secretion via release of
gonadotropin-releasing hormone (GnRH). In the present study, our aim was to
investigate whether kisspeptin has stimulatory effects on follicular development via
GnRH/gonadotropin secretion in cows. Japanese Black beef cows were intravenously
injected with full-length bovine kisspeptin [Kp-53 (0.2 or 2 nmol/kg)] or vehicle 5
days after they exhibited standing estrus (Day 0). In cows injected with Kp-53 at 2
nmol/kg, the follicular sizes of the first dominant follicles increased on Day 6 and
thereafter. Ovulation of the first dominant follicle occurred in 1 out of 4 cows
treated with Kp-53 at 2 nmol/kg. Injection of Kp-53 at 2 nmol/kg increased the
concentration of plasma luteinizing hormone (LH) but not follicle-stimulating
hormone, over a 4-h period following injection in all cows. The present study
suggests that administration of full-length kisspeptin causes LH secretion, which is
sustained for a few hours, and it is capable of stimulating follicular development
and/or ovulation. 相似文献
12.
实验探讨了大豆黄酮(DAI)对伊莎鸡卵泡发育及其芳香化酶(P450arom)mRNA表达的影响。实验选取16只产蛋后期伊莎鸡,等分为对照组和DAI处理组。对照组饲喂基础日粮,实验组在基础日粮中添加10 mg/kgDAI。实验持续7周后,分离排卵前卵泡(F1、F2、F3……)的颗粒层及小黄卵泡和大白卵泡,通过RT-PCR法检测P450arom mRNA表达的相对丰度。结果表明:DAI明显提高了伊莎鸡小黄卵泡和大白卵泡的数量,P450arommRNA在伊莎鸡不同发育阶段卵泡中的表达存在差异,部分卵泡P450arom mRNA表达的相对丰度显著增加。因此,在产蛋后期伊莎鸡基础日粮中添加DAI可增加不同发育阶段卵泡的数目,上调部分卵泡中与发育相关的基因表达以促进卵泡发育。 相似文献
13.
The aim of the present study was to examine the plasma concentrations of progesterone, oestradiol-17beta and cortisol in the cows with cystic follicle and to examine its relationship with the ovulatory response to gonadotropin-releasing hormone (GnRH). Eighty-five post-partum Holstein-Friesian cows with cystic follicles regardless of the presence of corpus luteum were studied. Follicular size, presence of corpus luteum and occurrence of ovulation were checked by palpation per rectum. Blood collection and palpation per rectum were conducted on days 0, 7 and 14. Gonadotropin-releasing hormone was administered at day 7. Plasma concentrations of progesterone, oestradiol-17beta and cortisol were determined. Progesterone concentrations of <3.2, 3.2-4.8 and >4.8 nmol/l were defined as low, intermediate and high, respectively. Sixty-three (74.1%) of 85 cows showed low (<3.2 nmol/l =1 ng/ml) progesterone concentrations on day 0. Only 40 (47.1%) of them showed low-low pattern of progesterone at days 0 and 7. In 27 (31.8%) of them, progesterone concentration had increased by day 14. Of 22 cows having high progesterone concentration (>/=4.8 nmol/l) on day 0, corpus luteum was not detected in 18 cows (21.2%). Only in 10 cows, cystic follicle disappeared after GnRH administration. However, only one of 27 cows in which progesterone pattern was low-low-high at days 0, 7 and 14 experienced ovulation of the cystic follicle. Significantly lower oestradiol-17beta concentration was found on day 7 in cows showing a low-low-low pattern than a low-low-high pattern of progesterone (43.0 +/- 4.6 vs 55.8 +/- 2.8 pmol/l, p < 0.05). There was no significant difference in cortisol concentration on any days (days 0, 7 and 14) between cows showing a low-low-low and low-low-high pattern of progesterone. These results suggest that approximately one-fifth of cows diagnosed to have ovarian cysts possess luteal cysts and that a high oestradiol-17beta concentration at the time of GnRH administration is involved in the subsequent ovulation of the follicle, although ovulated follicle may not be cystic. 相似文献
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15.
Sedqyar M Weng Q Watanabe G Kandiel MM Takahashi S Suzuki AK Taneda S Taya K 《The Journal of reproduction and development》2008,54(1):52-57
To clarify the cellular source and secretory pattern of inhibin in the Japanese quail during follicular development, the plasma concentrations of immunoreactive (ir) inhibin were measured from 1 to 7 weeks after hatching. Localization of the inhibin/activin alpha, beta A and beta B subunits was investigated by immunohistochemistry. To monitor development of the pituitary and ovarian functions, the plasma luteinizing hormone (LH) and progesterone concentrations were also measured. Ovarian weight increased gradually until 6 weeks of age and then abruptly increased at 7 weeks of age just at the onset of egg production. Plasma concentrations of LH increased significantly at 6 weeks of age. The plasma concentrations of ir-inhibin and progesterone and the pituitary contents of LH also increased significantly at 7 weeks of age. Immunohistochemically, the inhibin/activin alpha, beta A and beta B subunits were localized in the granulosa cells of all follicles during different stages of development from 1 to 7 weeks after hatching. The inhibin alpha, beta A and beta B subunits were also found in the interstitial cells but not theca cells of all follicles. These results demonstrated that the plasma concentrations of ir-inhibin of the female Japanese quails rose with ovarian development. The immunohistochemical results suggested that granulosa and interstitial cells are the major source of ovarian inhibins in female Japanese quails. 相似文献
16.
本试验旨在研究饲粮能量水平对后备母猪卵巢发育及卵巢促卵泡素受体(FSHR)和促黄体素受体(LH/CGR)mRNA表达的影响。选择27头体重(61.0±3.1)kg的长×大二元母猪,随机分成3组(每组3个重复,每个重复3头猪),分别饲喂低、中、高3个能量水平[分别为NRC(1998)推荐消化能需要的90%、100%和110%]的饲粮。试验期内各组平均日采食量相同,但摄入的消化能水平不同。试验母猪在第2个发情期的第19天屠宰。结果表明:高能组卵巢重及大卵泡数显著高于低能组(P0.05);各组间小卵泡数量差异不显著(P0.05)。高能组卵巢FSHR和LH/CGR mRNA表达量最高,显著高于低能组(P0.05)。由此得出,高能量水平饲粮可促进后备母猪卵巢发育,有利于促进卵巢FSHR和LH/CGR mRNA的表达。 相似文献
17.
D'Haeseleer M Cornillie P Simoens P van den Broeck W 《Anatomia, histologia, embryologia》2006,35(5):334-342
In the present study oestrogen receptor alpha(ERalpha) and oestrogen receptor beta (ERbeta) mRNA were localized in various ovarian cell types of 23 cows at different stages of the oestrous cycle. ERalpha was detected by immunohistochemistry and the localization of ERbeta mRNA was examined using in situ hybridization. The immunostaining of ERalpha was low in the ovarian follicles, tunica albuginea and surface epithelium, but high in cells of the deep stroma and superficial stroma, which indicates a functional role of ERalpha in the cells surrounding the follicles. In contrast, ERbeta mRNA scores were low to moderate in primordial and primary follicles, and increased with the development of the follicle. ERbeta mRNA scores were higher in cystic follicles than in obliterative follicles. In the corpora lutea and corpora albicantia the scores for ERbeta mRNA were moderate. Furthermore, in the corpora lutea, ERbeta mRNA levels showed cyclic variations and were low during early dioestrus. The correlation between plasma progesterone levels and the score for ER was low and negative in all ovarian cell types. This study demonstrates the predominant role of ERbeta over ERalpha in bovine ovarian structures. Furthermore, the colocalization of both ERbeta mRNA and ERalpha in most cell types suggests possible interactions between both ER subtypes. 相似文献
18.
Ovarian follicular vasculature is involved in follicular development and ovulation. Angiopoietin (ANPT)-Tie system is important for vascularization of the tissue surrounding the developing follicles and corpus luteum (CL). To determine how the expression of ANPT-1, ANPT-2 and their receptors in the follicles would be associated with the ovulatory process, the present study was conducted to examine mRNA expressions of ANPT-1, ANPT-2 and their receptors during the periovulatory phase in gonadotrophin-releasing hormone (GnRH)-treated cows. The ovaries were collected by transvaginal ovariectomy (n = 5, cows/group) and the follicles (n = 5, one follicle/cow) were classified into following groups: before GnRH administration [before luteinizing hormone (LH) surge]; 3-5 h after GnRH (during LH surge); 10 h after GnRH; 20 h after GnRH; 25 h after GnRH (peri-ovulation); and early CL (days 2-3). The mRNA expression was analysed by quantitative real-time PCR (rotor-gene 3000). Angiopoietin-1 expression rapidly decreased at 3-5 h and kept low level at 10 h after GnRH treatment compared with that before GnRH, and returned to the level before LH surge in the follicles >20 h after GnRH treatment. The levels of ANPT-2 mRNA decreased at 10 and 25 h after treatment compared with other periods. The ratio of ANPT-2/ANPT-1 (an index for destabilization of blood vessels) increased in the follicles at 3-5 h after GnRH treatment only. Both of Tie-1 and Tie-2 receptor expressions decreased in the follicles at 25 h after GnRH treatment. The results of the present study indicated that mRNA expressions of ANPT-1, ANPT-2 and their receptors changed in the bovine follicles during periovulatory period. These results suggest that angiopoietin-Tie system is associated with the initiation of vasculature of follicle that grows towards ovulation. 相似文献